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Literature summary extracted from
- Bile salt sulfotransferase in guinea pig liver (1982), Biochim. Biophys. Acta, 717, 316-321.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 2.8.2.14 | freezing and thawing results in significant loss in activity, substantial stabilization with addition of sucrose | Cavia porcellus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.8.2.14 | 7,12-diketochenodeoxycholate | - |
Cavia porcellus |  |
| 2.8.2.14 | adenosine-3',5'-diphosphate | - |
Cavia porcellus | |
| 2.8.2.14 | ATP | - |
Cavia porcellus | |
| 2.8.2.14 | EDTA | - |
Cavia porcellus | |
| 2.8.2.14 | iodoacetate | - |
Cavia porcellus | |
| 2.8.2.14 | NaF | - |
Cavia porcellus | |
| 2.8.2.14 | NaN3 | - |
Cavia porcellus | |
| 2.8.2.14 | p-chloromercuribenzoate | - |
Cavia porcellus | |
| 2.8.2.14 | Taurodehydrocholate | - |
Cavia porcellus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.8.2.14 | 0.0014 | - |
3'-phosphoadenylylsulfate | pH 6.5, 37°C | Cavia porcellus | |
| 2.8.2.14 | 0.077 | - |
taurolithocholate | pH 6.5, 37°C | Cavia porcellus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.8.2.14 | CaCl2 | activity 107% | Cavia porcellus | |
| 2.8.2.14 | CuCl2 | activity 136% | Cavia porcellus | |
| 2.8.2.14 | CuSO4 | activity 107% | Cavia porcellus | |
| 2.8.2.14 | FeSO4 | activity 134% | Cavia porcellus | |
| 2.8.2.14 | KCl | activity 128% | Cavia porcellus | |
| 2.8.2.14 | LiCl | activity 108% | Cavia porcellus | |
| 2.8.2.14 | Mg2+ | required for activity | Cavia porcellus | |
| 2.8.2.14 | ZnSO4 | activity 111% | Cavia porcellus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.8.2.14 | 76000 | - |
gel filtration | Cavia porcellus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.8.2.14 | 3'-phosphoadenylyl sulfate + taurolithocholate | Cavia porcellus | - |
adenosine 3',5'-bisphosphate + taurolithocholate sulfate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.8.2.14 | Cavia porcellus | - |
guinea pig | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.8.2.14 | - |
Cavia porcellus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 2.8.2.14 | liver | - |
Cavia porcellus | - |
| 2.8.2.14 | additional information | no activity in kidney | Cavia porcellus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.8.2.14 | 0.0216 | - |
- |
Cavia porcellus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 2.8.2.14 | -20°C, (NH4)2SO4 precipitate, stable for several months | Cavia porcellus |
| 2.8.2.14 | 4°C, loses about 50% activity in 1 week | Cavia porcellus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.8.2.14 | 3'-phosphoadenylyl sulfate + isolithocholic acid | 5beta-cholanoic acid-3beta-ol | Cavia porcellus | adenosine 3',5'-bisphosphate + isolithocholate sulfate | - |
? | |
| 2.8.2.14 | 3'-phosphoadenylyl sulfate + taurolithocholate | - |
Cavia porcellus | adenosine 3',5'-bisphosphate + taurolithocholate sulfate | - |
? | |
| 2.8.2.14 | 3'-phosphoadenylyl-sulfate + 5-cholenic acid-3beta-ol | - |
Cavia porcellus | adenosine 3',5'-bisphosphate + 5-cholenic acid-3-beta-ol sulfate | - |
? | |
| 2.8.2.14 | 3'-phosphoadenylyl-sulfate + 5alpha-cholanoic acid-3beta-ol | - |
Cavia porcellus | adenosine 3',5'-bisphosphate + 5alpha-cholanic acid-3beta-ol sulfate | - |
? | |
| 2.8.2.14 | 3'-phosphoadenylyl-sulfate + glycochenodeoxycholate | - |
Cavia porcellus | adenosine 3',5'-bisphosphate + glycochenodeoxycholate monosulfate | - |
? | |
| 2.8.2.14 | 3'-phosphoadenylyl-sulfate + glycocholate | - |
Cavia porcellus | adenosine 3',5'-bisphosphate + glycocholate 3-sulfate | - |
? | |
| 2.8.2.14 | 3'-phosphoadenylyl-sulfate + glycolithocholate | - |
Cavia porcellus | adenosine 3',5'-bisphosphate + glycolithocholate 3-sulfate | - |
? | |
| 2.8.2.14 | 3'-phosphoadenylyl-sulfate + lithocholate | - |
Cavia porcellus | adenosine 3',5'-bisphosphate + lithocholate 3-sulfate | - |
? | |
| 2.8.2.14 | 3'-phosphoadenylyl-sulfate + taurochenodeoxycholate | - |
Cavia porcellus | adenosine 3',5'-bisphosphate + taurochenodeoxycholate monosulfate + taurochenodeoxycholate disulfate | - |
? | |
| 2.8.2.14 | 3'-phosphoadenylyl-sulfate + taurocholate | - |
Cavia porcellus | adenosine 3',5'-bisphosphate + taurocholate monosulfate + taurocholate disulfate + taurocholate trisulfate | - |
? | |
| 2.8.2.14 | additional information | purified enzyme does not react with estrone, estradiol, testosterone, dehydroepiandrosterone, cholesterol, phenol, tyramine, and serotonin | Cavia porcellus | ? | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.8.2.14 | 6.8 | - |
- |
Cavia porcellus |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 2.8.2.14 | Cavia porcellus | - |
5.9 | 5.6 |
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