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Literature summary extracted from

  • Foldes, A.; Meek, J.L.
    Rat brain phenolsulfotransferase: partial purification and some properties (1973), Biochim. Biophys. Acta, 327, 365-374.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.1 0.01
-
dihydroxyphenylacetic acid pH 5.8, 37°C Rattus norvegicus
2.8.2.1 0.014
-
homovanillic acid pH 5.8, 37°C Rattus norvegicus
2.8.2.1 0.017
-
3,4-dihydroxyphenylglycol pH 6.0, 37°C Rattus norvegicus
2.8.2.1 0.018
-
3-methoxy-4-hydroxyphenylglycol pH 6.4, 37°C Rattus norvegicus
2.8.2.1 0.02
-
dopamine pH 9.0, 37°C Rattus norvegicus
2.8.2.1 0.04
-
phenol pH 5.8, 37°C Rattus norvegicus
2.8.2.1 0.1
-
vanillylmandelic acid pH 6.2, 37°C Rattus norvegicus
2.8.2.1 0.1
-
norepinephrine pH 9.0, 37°C Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.1 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.1 30fold from brain Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.1 brain
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.1 additional information
-
-
Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.8.2.1 -20°C, 7 days, 20% loss of activity of partially purified enzyme Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.1 3'-phosphoadenylyl sulfate + a phenol
-
Rattus norvegicus adenosine 3',5'-bisphosphate + an aryl sulfate
-
?
2.8.2.1 3'-phosphoadenylylsulfate + 3,4-dihydroxyphenylacetic acid
-
Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
?
2.8.2.1 3'-phosphoadenylylsulfate + 3,4-dihydroxyphenylglycol
-
Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
?
2.8.2.1 3'-phosphoadenylylsulfate + 3-methoxy-4-hydroxyphenylglycol
-
Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
?
2.8.2.1 3'-phosphoadenylylsulfate + dopamine low activity Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
?
2.8.2.1 3'-phosphoadenylylsulfate + homovanillic acid
-
Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
?
2.8.2.1 3'-phosphoadenylylsulfate + norepinephrine
-
Rattus norvegicus adenosine 3',5'-bisphosphate + norepinephrine sulfate
-
?
2.8.2.1 3'-phosphoadenylylsulfate + phenol
-
Rattus norvegicus adenosine 3',5'-bisphosphate + phenyl sulfate
-
?
2.8.2.1 3'-phosphoadenylylsulfate + vanillylmandelic acid
-
Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.1 37
-
assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.1 5.8
-
-
Rattus norvegicus
2.8.2.1 6
-
with substrate 3,4-dihydroxyphenylglycol Rattus norvegicus
2.8.2.1 6.2
-
with substrate vanillylmandelic acid Rattus norvegicus
2.8.2.1 6.4
-
with substrate 3-methoxy-4-hydroxyphenylglycol Rattus norvegicus
2.8.2.1 9
-
with substrate dopamine or norepinephrine Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.8.2.1 6
-
4°C, 82 h, 60% loss of activity Rattus norvegicus
2.8.2.1 8
-
4°C, 82 h, 80% loss of activity Rattus norvegicus