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Literature summary extracted from

  • Benovic, J.L.; Mayor, F.; Staniczewski, C.; Lefkowitz, R.J.; Caron, M.G.
    Purification and characterization of the beta-adrenergic receptor kinase (1987), J. Biol. Chem., 262, 9026-9032.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.15 isoproterenol requirement, beta-agonist, beta-adrenergic receptor as substrate Bos taurus
2.7.11.15 additional information not effected by Ca2+ or Co2+ Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.15 Digitonin 0.05-0.1%, 93-95% inhibition Bos taurus
2.7.11.15 additional information not affected by Ca2+ or Co2+ Bos taurus
2.7.11.15 NaCl 20 mM, 48% inhibition, as efficient as F-, I-, NO2- or acetate, less efficient than citrate, phosphate or sulfate Bos taurus
2.7.11.15 NaF as efficient as Cl-, I-, NO2- or acetate, less efficient than citrate, phosphate or sulfate Bos taurus
2.7.11.15 NaI as efficient as Cl-, F-, NO2- or acetate, less efficient than citrate, phosphate or sulfate Bos taurus
2.7.11.15 NaNO2 as efficient as Cl-, I-, F- or acetate, less efficient than citrate, phosphate or sulfate Bos taurus
2.7.11.15 Sodium acetate as efficient as Cl-, I-, NO2- or F-, less efficient than citrate, phosphate or sulfate Bos taurus
2.7.11.15 Sodium citrate 20 mM, 97% inhibition, more efficient than phosphate, sulfate, Cl-, F-, I-, NO2- or acetate Bos taurus
2.7.11.15 Sodium phosphate 20 mM, 76% inhibition, less efficient than citrate, as good as sulfate, more efficient than Cl-, F-, I-, NO2- or acetate Bos taurus
2.7.11.15 Sodium sulfate less efficient than citrate, as good as phosphate, more efficient than Cl-, F-, I-, NO2- or acetate Bos taurus
2.7.11.15 Zn2+ 0.1-0.2 mM ZnCl2, 94-98% inhibition Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.15 0.00025
-
 beta-adrenergic receptor pH 7.5, 30°C Bos taurus
2.7.11.15 0.006
-
 rhodopsin pH 7.5, 30°C Bos taurus
2.7.11.15 0.033
-
 ATP pH 7.5, 30°C, beta-adrenergic receptor as substrate Bos taurus
2.7.11.15 0.037
-
 ATP pH 7.5, 30°C, rhodopsin as substrate Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.15 cytosol
-
 Bos taurus 5829
-
2.7.11.15 plasma membrane beta-AR may serve as membrane anchor for enzyme, translocation of beta-ARK from the cytosol to the plasma membrane Bos taurus 5886
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.15 Mg2+ requirement Bos taurus
2.7.11.15 Mg2+ optimal concentration: 2-6 mM Bos taurus
2.7.11.15 Mn2+ requirement, can replace Mg2+ with 50% efficiency, optimal concentration: 1-3 mM Bos taurus
2.7.11.15 additional information not activated by Ca2+, Co2+ or Zn2+ Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.15 80000
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.15 ATP + beta-adrenergic receptor Bos taurus agonist-occupied form of the receptor ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor Bos taurus involved in homologous desensitization of beta-adrenergic receptor ADP + phospho-beta-adrenergic receptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.15 Bos taurus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.15 phosphoprotein enzyme appears to autophosphorylate Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.15 20300fold, to near homogeneity Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.15 brain cerebral cortex Bos taurus
-
2.7.11.15 brain highest beta-ARK activity in cerebral cortex Bos taurus
-
2.7.11.15 cerebral cortex highest beta-ARK activity Bos taurus
-
2.7.11.15 additional information tissue distribution Bos taurus
-
2.7.11.15 additional information ubiquitous enzyme Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.15 0.00873
-
 pH 7.5, 30°C Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.7.11.15 4°C, crude enzyme: several months, stable, purified enzyme: t1/2 of 5-10 days Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.15 ATP + beta-adrenergic receptor incorporation of about 9 mol phosphate/mol receptor Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor specifically phosphorylates the agonist-occupied form of the receptor Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor beta-AR from hamster lung Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor beta-AR is a much better substrate than rhodopsin Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor agonist-occupied form of the receptor Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor involved in homologous desensitization of beta-adrenergic receptor Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + rhodopsin beta-AR is a much better substrate than rhodopsin Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.15 ATP + rhodopsin light-dependent, actual substrate: light-bleached rhodopsin Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.15 ATP + rhodopsin incorporation of 0.15 mol phosphate/mol rhodopsin Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.15 ATP + rhodopsin in form of bovine rod outer segments Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.15 GTP + beta-adrenergic receptor agonist-occupied form of beta-AR from hamster lung, GTP can substitute ATP, 2% as effective as ATP Bos taurus GDP + phospho-beta-adrenergic receptor
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.15 monomer 1 * 80000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.15 30
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.15 6 7.5
-
 Bos taurus
2.7.11.15 7.5
-
 assay at Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.15 ATP requirement Bos taurus