Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Smith, S.T.; Rajagopalan, K.V.; Handler, P.
    Purification and properties of xanthine dehydrogenase from Micrococcus lactilyticus (1967), J. Biol. Chem., 242, 4108-4117.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.1.4 Ferredoxin 26fold increase of cytochrome c reduction, 2fold increase of oxygen reduction Veillonella atypica

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.1.4 8-Azaxanthine 50% inhibition of ferricyanide reduction in xanthine oxidation assay at 5 mM Veillonella atypica
1.17.1.4 KCN 10-50% inhibition of xanthine oxidation only in the presence of Tris or phosphate buffers from 0.01 to 0.1 M inhibitor concentration Veillonella atypica
1.17.1.4 methanol develops inhibition during course of catalysis, enhanced inhibition in the presence of ferricyanide in the oxygen-dependent oxidation of xanthine Veillonella atypica
1.17.1.4 Urate 50% inhibition of xanthine oxidation at 0.5 mM Veillonella atypica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.1.4 Fe
-
Veillonella atypica
1.17.1.4 Mo
-
Veillonella atypica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.1.4 250000
-
sucrose density gradient centrifugation Veillonella atypica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.1.4 6,8-dihydroxypurine + O2 Veillonella atypica
-
? + H2O2
-
?
1.17.1.4 hypoxanthine + O2 Veillonella atypica
-
xanthine + H2O2
-
?
1.17.1.4 hypoxanthine + urate Veillonella atypica oxygen-free assay xanthine + 6,8-dihydroxypurine
-
r
1.17.1.4 purine + NAD+ + H2O Veillonella atypica
-
8-hydroxypurine + NADH + H+
-
?
1.17.1.4 xanthine + O2 Veillonella atypica dismutation reaction hypoxanthine + ?
-
?
1.17.1.4 xanthine + O2 + H2O Veillonella atypica presence of ferredoxin enhances rate of oxygen reduction urate + O2- + 2 H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.4 Veillonella atypica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.4 to homogeneity, precipitation and chromatography techniques Veillonella atypica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.1.4 cell culture
-
Veillonella atypica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.1.4 0.08
-
-
Veillonella atypica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.4 2,6-dichloroindophenol + NADH + H+
-
Veillonella atypica reduced 2,6-dichlorophenolindophenol + NAD+
-
r
1.17.1.4 6,8-dihydroxypurine + O2
-
Veillonella atypica ? + H2O2
-
?
1.17.1.4 6,8-dihydroxypurine + O2 + H2O
-
Veillonella atypica ? + H2O2
-
?
1.17.1.4 9-methylhypoxanthine + O2
-
Veillonella atypica 9-methylxanthine + H2O2
-
?
1.17.1.4 benzaldehyde + O2
-
Veillonella atypica benzoate + H2O2
-
?
1.17.1.4 hypoxanthine + O2
-
Veillonella atypica xanthine + H2O2
-
?
1.17.1.4 hypoxanthine + urate oxygen-free assay Veillonella atypica xanthine + 6,8-dihydroxypurine
-
r
1.17.1.4 purine + NAD+ + H2O
-
Veillonella atypica 8-hydroxypurine + NADH + H+
-
?
1.17.1.4 salicylaldehyde + O2
-
Veillonella atypica salicylate + H2O2
-
r
1.17.1.4 xanthine + cytochrome c + H2O presence of ferredoxin enhances cytochrom c reduction Veillonella atypica urate + reduced cytochrome c
-
?
1.17.1.4 xanthine + ferredoxin + H2O
-
Veillonella atypica urate + reduced ferredoxin
-
?
1.17.1.4 xanthine + ferricyanide + H2O
-
Veillonella atypica urate + ferrocyanide
-
?
1.17.1.4 xanthine + methylene blue + H2O
-
Veillonella atypica urate + reduced methylene blue
-
?
1.17.1.4 xanthine + myoglobin + H2O
-
Veillonella atypica urate + reduced myoglobin
-
?
1.17.1.4 xanthine + nitroblue tetrazolium + H2O
-
Veillonella atypica urate + ?
-
?
1.17.1.4 xanthine + O2 dismutation reaction Veillonella atypica hypoxanthine + ?
-
?
1.17.1.4 xanthine + O2 + H2O presence of ferredoxin enhances rate of oxygen reduction Veillonella atypica urate + O2- + 2 H+
-
?
1.17.1.4 xanthine + phenazine methosulfate + H2O
-
Veillonella atypica urate + ?
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.17.1.4 0.1
-
Salicylaldehyde electron acceptor: oxygen Veillonella atypica
1.17.1.4 0.7
-
purine electron acceptor: oxygen Veillonella atypica
1.17.1.4 0.717
-
cytochrome c cosubstrate xanthine Veillonella atypica
1.17.1.4 0.75
-
hypoxanthine electron acceptor: oxygen Veillonella atypica
1.17.1.4 0.8
-
oxygen cosubstrate xanthine Veillonella atypica
1.17.1.4 2.5
-
xanthine electron acceptor: oxygen Veillonella atypica
1.17.1.4 5.67
-
2,6-dichlorophenolindophenol cosubstrate xanthine Veillonella atypica
1.17.1.4 9.55
-
Nitro blue tetrazolium cosubstrate xanthine Veillonella atypica
1.17.1.4 18.2
-
ferricyanide cosubstrate xanthine Veillonella atypica
1.17.1.4 20.2
-
Ferredoxin cosubstrate xanthine Veillonella atypica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.1.4 6.5
-
xanthine oxidation, presence of oxygen, two pH-optima Veillonella atypica
1.17.1.4 9.5
-
xanthine oxidation, presence of oxygen, two pH-optima Veillonella atypica

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.17.1.4 7
-
unstable below, stabilized and activated at higher pH values Veillonella atypica

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.4 FAD
-
Veillonella atypica
1.17.1.4 additional information molybdoironflavoprotein: 8 : 8 : 2 : 1.5 ratio of iron to sulfide to flavin to molybdenum Veillonella atypica