EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.17.1.4 | Iron | contains FeSI and FeSII clusters | Pseudomonas putida | |
1.17.1.4 | Iron-sulfur-center | - |
Pseudomonas putida | |
1.17.1.4 | Molybdenum | contains molybdenum-molybdopterin | Pseudomonas putida | |
1.17.1.4 | molybdenum-center | - |
Pseudomonas putida |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.17.1.4 | 46238 | - |
2 * 91041 + 2 * 46238, MALDI-TOF mass spectrometry | Pseudomonas putida |
1.17.1.4 | 51000 | - |
2 * 94000 + 2 * 51000, SDS-PAGE | Pseudomonas putida |
1.17.1.4 | 91041 | - |
2 * 91041 + 2 * 46238, MALDI-TOF mass spectrometry | Pseudomonas putida |
1.17.1.4 | 94000 | - |
2 * 94000 + 2 * 51000, SDS-PAGE | Pseudomonas putida |
1.17.1.4 | 580000 | - |
SDS-PAGE | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.17.1.4 | hypoxanthine + NAD+ + H2O | Pseudomonas putida | - |
xanthine + NADH + H+ | - |
? | |
1.17.1.4 | xanthine + NAD+ + H2O | Pseudomonas putida | - |
urate + NADH | - |
? | |
1.17.1.4 | xanthine + O2 + H2O | Pseudomonas putida | 4% of the activity compared to xanthine-NAD+ | urate + O2- + 2 H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.17.1.4 | Pseudomonas putida | - |
- |
- |
1.17.1.4 | Pseudomonas putida 86 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.17.1.4 | - |
Pseudomonas putida |
1.17.1.4 | ammonium sulfate precipitation, phenyl-Sepharose column chromatography, DEAE-10 Macro-Prep MP10 column chromatography, and Bio-Prep SE-1000/17 column chromatography | Pseudomonas putida |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.17.1.4 | cell culture | inducible by hypoxanthine | Pseudomonas putida | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.17.1.4 | 0.9 | - |
crude extract | Pseudomonas putida |
1.17.1.4 | 26.7 | - |
hypoxanthine-NAD+ assay | Pseudomonas putida |
1.17.1.4 | 35.2 | - |
after 39.1fold purification | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.17.1.4 | 3,4-dihydroxybenzaldehyde + NAD+ + H2O | 1.2% activity compared to xanthine | Pseudomonas putida | 3,4-dihydroxybenzoate + NADH + H+ | - |
? | |
1.17.1.4 | 3,4-dihydroxybenzaldehyde + NAD+ + H2O | 1.2% activity compared to xanthine | Pseudomonas putida 86 | 3,4-dihydroxybenzoate + NADH + H+ | - |
? | |
1.17.1.4 | 4-dimethylaminobenzaldehyde + NAD+ + H2O | 0.3% activity compared to xanthine | Pseudomonas putida | 4-dimethylaminobenzoate + NADH | - |
? | |
1.17.1.4 | 4-hydroxybenzaldehyde + NAD+ + H2O | 0.7% activity compared to xanthine | Pseudomonas putida | 4-hydroxybenzoate + NADH + H+ | - |
? | |
1.17.1.4 | 4-hydroxybenzaldehyde + NAD+ + H2O | 0.7% activity compared to xanthine | Pseudomonas putida 86 | 4-hydroxybenzoate + NADH + H+ | - |
? | |
1.17.1.4 | benzaldehyde + NAD+ + H2O | 1.3% activity compared to xanthine | Pseudomonas putida | benzoate + NADH + H+ | - |
? | |
1.17.1.4 | benzaldehyde + NAD+ + H2O | 1.3% activity compared to xanthine | Pseudomonas putida 86 | benzoate + NADH + H+ | - |
? | |
1.17.1.4 | hypoxanthine + ferricyanide + H2O | - |
Pseudomonas putida | xanthine + ferrocyanide | - |
? | |
1.17.1.4 | hypoxanthine + ferricyanide + H2O | - |
Pseudomonas putida 86 | xanthine + ferrocyanide | - |
? | |
1.17.1.4 | hypoxanthine + NAD+ + H2O | - |
Pseudomonas putida | xanthine + NADH + H+ | - |
? | |
1.17.1.4 | hypoxanthine + NAD+ + H2O | 149% activity compared to xanthine | Pseudomonas putida | urate + ? + NADH | - |
? | |
1.17.1.4 | additional information | NAD+ is the most effcient electron acceptor, followed by 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl-2H-tetrazolium chloride and ferricyanide | Pseudomonas putida | ? | - |
? | |
1.17.1.4 | additional information | NAD+ is the most effcient electron acceptor, followed by 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl-2H-tetrazolium chloride and ferricyanide | Pseudomonas putida 86 | ? | - |
? | |
1.17.1.4 | phthalazine + NAD+ + H2O | 0.4% activity compared to xanthine | Pseudomonas putida | 1-(2H)-phthalazinone + NADH | - |
? | |
1.17.1.4 | purine + NAD+ + H2O | 5% activity compared to xanthine | Pseudomonas putida | 8-hydroxypurine + NADH + H+ | - |
? | |
1.17.1.4 | quinazoline + NAD+ + H2O | 2% activity compared to xanthine | Pseudomonas putida | 4-(3H)-quinazolinone + NADH | - |
? | |
1.17.1.4 | xanthine + ferricyanide + H2O | 58% of the activity compared to xanthine-NAD+ | Pseudomonas putida | urate + ferrocyanide | - |
? | |
1.17.1.4 | xanthine + NAD+ + H2O | - |
Pseudomonas putida | urate + NADH | - |
? | |
1.17.1.4 | xanthine + NAD+ + H2O | 100% activity | Pseudomonas putida | urate + NADH | - |
? | |
1.17.1.4 | xanthine + O2 + H2O | 4% of the activity compared to xanthine-NAD+ | Pseudomonas putida | urate + O2- + 2 H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.17.1.4 | heterooctamer | 2 * 91041 + 2 * 46238, MALDI-TOF mass spectrometry | Pseudomonas putida |
1.17.1.4 | heterooctamer | 2 * 94000 + 2 * 51000, SDS-PAGE | Pseudomonas putida |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.17.1.4 | XDH | - |
Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.17.1.4 | FAD | - |
Pseudomonas putida | |
1.17.1.4 | additional information | cofactor compostion similar to eukaryotic enzymes | Pseudomonas putida | |
1.17.1.4 | NAD+ | - |
Pseudomonas putida | |
1.17.1.4 | NADP+ | - |
Pseudomonas putida |