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Literature summary extracted from

  • Orsi, B.A.; Spencer, B.
    Choline sulphokinase (sulfotransferase) (1964), J. Biochem., 56, 81-91.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.8.2.6 ethanol
-
Aspergillus nidulans
2.8.2.6 glycol
-
Aspergillus nidulans
2.8.2.6 n-Propanol maximal activation, at 2.5 mM, inhibition above 25 mM Aspergillus nidulans

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.6 1-Naphthylamine
-
Aspergillus nidulans
2.8.2.6 2,4-dinitrophenol
-
Aspergillus nidulans
2.8.2.6 3-Aminophenol
-
Aspergillus nidulans
2.8.2.6 4-Dimethylaminophenol
-
Aspergillus nidulans
2.8.2.6 4-nitrophenol competitive inhibition Aspergillus nidulans
2.8.2.6 Acetylcholine
-
Aspergillus nidulans
2.8.2.6 beta-homocholine
-
Aspergillus nidulans
2.8.2.6 carnitine competitive inhibition Aspergillus nidulans
2.8.2.6 Co2+ sensitive to metal ions, almost complete inhibition at 6.0 mM Aspergillus nidulans
2.8.2.6 cyanide
-
Aspergillus nidulans
2.8.2.6 diethanolamine
-
Aspergillus nidulans
2.8.2.6 Dimethylaminoethanethiol
-
Aspergillus nidulans
2.8.2.6 dimethylaminoethanol substrate inhibition Aspergillus nidulans
2.8.2.6 Dimethylaminopropan-1-ol competitive inhibition Aspergillus nidulans
2.8.2.6 Dimethylaminopropen-1-ol
-
Aspergillus nidulans
2.8.2.6 dimethylethylaminoethanol substrate inhibition Aspergillus nidulans
2.8.2.6 ethyltrimethylammonium bromide
-
Aspergillus nidulans
2.8.2.6 Fe3+ sensitive to metal ions, almost complete inhibition at 6.0 mM Aspergillus nidulans
2.8.2.6 gamma-homocholine
-
Aspergillus nidulans
2.8.2.6 hexadecyltrimethylammonium bromide
-
Aspergillus nidulans
2.8.2.6 Mn2+ sensitive to metal ions, almost complete inhibition at 6.0 mM Aspergillus nidulans
2.8.2.6 additional information iodoacetate is less effective as inhibitor, di-isopropylfluorophosphate is without action Aspergillus nidulans
2.8.2.6 N-ethylmaleimide
-
Aspergillus nidulans
2.8.2.6 n-Propanol concentrations above 0.025 M Aspergillus nidulans
2.8.2.6 Ni2+ sensitive to metal ions, almost complete inhibition at 6.0 mM Aspergillus nidulans
2.8.2.6 p-chloromercuribenzoate
-
Aspergillus nidulans
2.8.2.6 phenol strong inhibitor Aspergillus nidulans
2.8.2.6 Prostigmine
-
Aspergillus nidulans
2.8.2.6 sulfate
-
Aspergillus nidulans
2.8.2.6 thiocholine
-
Aspergillus nidulans
2.8.2.6 triethanolamine
-
Aspergillus nidulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.6 0.0222
-
3'-phosphoadenylylsulfate pH 7.3, 37°C Aspergillus nidulans
2.8.2.6 12
-
choline pH 7.3, 37°C Aspergillus nidulans
2.8.2.6 20
-
N,N-dimethylethylaminoethanol pH 7.3, 37°C Aspergillus nidulans
2.8.2.6 25
-
dimethylaminoethanol pH 7.3, 37°C Aspergillus nidulans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.2.6 additional information not even a partial requirement for Mg2+ Aspergillus nidulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.6 3'-phosphoadenylylsulfate + choline Aspergillus nidulans
-
adenosine 3',5'-bisphosphate + choline sulfate
-
ir

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.6 Aspergillus nidulans
-
CMI 38,595 S eta, sulfateless mutant
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.6 partial Aspergillus nidulans

Storage Stability

EC Number Storage Stability Organism
2.8.2.6 -20°C, freezing and storing for 3 days results in complete loss of activity Aspergillus nidulans
2.8.2.6 2°C, more stable after (NH4)2SO4 or acetone fractionation, activity is reduced by 85% during 4 weeks Aspergillus nidulans
2.8.2.6 2°C, very unstable, loses 95% of its activity after 5 days Aspergillus nidulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.6 3'-phosphoadenylylsulfate + choline
-
Aspergillus nidulans adenosine 3',5'-bisphosphate + choline sulfate
-
ir
2.8.2.6 3'-phosphoadenylylsulfate + choline sulfate transfer appears to be irreversible in vitro Aspergillus nidulans adenosine 3',5'-bisphosphate + choline sulfate
-
ir
2.8.2.6 3'-phosphoadenylylsulfate + N,N-dimethylaminoethanol
-
Aspergillus nidulans adenosine 3',5'-bisphosphate + N,N-dimethylaminoethanol O-sulfate
-
?
2.8.2.6 3'-phosphoadenylylsulfate + N,N-dimethylethylaminoethanol
-
Aspergillus nidulans adenosine 3',5'-bisphosphate + N,N-dimethylethylaminoethanol O-sulfate
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.6 20 30
-
Aspergillus nidulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.8.2.6 46
-
no loss of activity during 10 min at pH 9.2, 30% loss at pH 7.3, 34% loss at pH 5.0 Aspergillus nidulans
2.8.2.6 55
-
almost completely inactivated Aspergillus nidulans
2.8.2.6 58.5
-
complete loss of activity at pH 5.0, 85% activity retained at pH 9.2 Aspergillus nidulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.6 7.8
-
-
Aspergillus nidulans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.8.2.6 5 10 about 50% of activity maximum at pH 5.8 and 10.1 Aspergillus nidulans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.8.2.6 4.5 12 stable at room temperature in the pH range 8.0-11.0, above pH 11.0 enzyme is rapidly inactivated, 80% inactivation at pH 12.0, below pH 8.0 stablity declines slowly with decreasing pH, maximum stability at pH 8,5-10.5 Aspergillus nidulans