EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.8.2.6 | ethanol | - |
Aspergillus nidulans | |
2.8.2.6 | glycol | - |
Aspergillus nidulans | |
2.8.2.6 | n-Propanol | maximal activation, at 2.5 mM, inhibition above 25 mM | Aspergillus nidulans |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.8.2.6 | 1-Naphthylamine | - |
Aspergillus nidulans | |
2.8.2.6 | 2,4-dinitrophenol | - |
Aspergillus nidulans | |
2.8.2.6 | 3-Aminophenol | - |
Aspergillus nidulans | |
2.8.2.6 | 4-Dimethylaminophenol | - |
Aspergillus nidulans | |
2.8.2.6 | 4-nitrophenol | competitive inhibition | Aspergillus nidulans | |
2.8.2.6 | Acetylcholine | - |
Aspergillus nidulans | |
2.8.2.6 | beta-homocholine | - |
Aspergillus nidulans | |
2.8.2.6 | carnitine | competitive inhibition | Aspergillus nidulans | |
2.8.2.6 | Co2+ | sensitive to metal ions, almost complete inhibition at 6.0 mM | Aspergillus nidulans | |
2.8.2.6 | cyanide | - |
Aspergillus nidulans | |
2.8.2.6 | diethanolamine | - |
Aspergillus nidulans | |
2.8.2.6 | Dimethylaminoethanethiol | - |
Aspergillus nidulans | |
2.8.2.6 | dimethylaminoethanol | substrate inhibition | Aspergillus nidulans | |
2.8.2.6 | Dimethylaminopropan-1-ol | competitive inhibition | Aspergillus nidulans | |
2.8.2.6 | Dimethylaminopropen-1-ol | - |
Aspergillus nidulans | |
2.8.2.6 | dimethylethylaminoethanol | substrate inhibition | Aspergillus nidulans | |
2.8.2.6 | ethyltrimethylammonium bromide | - |
Aspergillus nidulans | |
2.8.2.6 | Fe3+ | sensitive to metal ions, almost complete inhibition at 6.0 mM | Aspergillus nidulans | |
2.8.2.6 | gamma-homocholine | - |
Aspergillus nidulans | |
2.8.2.6 | hexadecyltrimethylammonium bromide | - |
Aspergillus nidulans | |
2.8.2.6 | Mn2+ | sensitive to metal ions, almost complete inhibition at 6.0 mM | Aspergillus nidulans | |
2.8.2.6 | additional information | iodoacetate is less effective as inhibitor, di-isopropylfluorophosphate is without action | Aspergillus nidulans | |
2.8.2.6 | N-ethylmaleimide | - |
Aspergillus nidulans | |
2.8.2.6 | n-Propanol | concentrations above 0.025 M | Aspergillus nidulans | |
2.8.2.6 | Ni2+ | sensitive to metal ions, almost complete inhibition at 6.0 mM | Aspergillus nidulans | |
2.8.2.6 | p-chloromercuribenzoate | - |
Aspergillus nidulans | |
2.8.2.6 | phenol | strong inhibitor | Aspergillus nidulans | |
2.8.2.6 | Prostigmine | - |
Aspergillus nidulans | |
2.8.2.6 | sulfate | - |
Aspergillus nidulans | |
2.8.2.6 | thiocholine | - |
Aspergillus nidulans | |
2.8.2.6 | triethanolamine | - |
Aspergillus nidulans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.8.2.6 | 0.0222 | - |
3'-phosphoadenylylsulfate | pH 7.3, 37°C | Aspergillus nidulans | |
2.8.2.6 | 12 | - |
choline | pH 7.3, 37°C | Aspergillus nidulans | |
2.8.2.6 | 20 | - |
N,N-dimethylethylaminoethanol | pH 7.3, 37°C | Aspergillus nidulans | |
2.8.2.6 | 25 | - |
dimethylaminoethanol | pH 7.3, 37°C | Aspergillus nidulans |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.8.2.6 | additional information | not even a partial requirement for Mg2+ | Aspergillus nidulans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.2.6 | 3'-phosphoadenylylsulfate + choline | Aspergillus nidulans | - |
adenosine 3',5'-bisphosphate + choline sulfate | - |
ir |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.8.2.6 | Aspergillus nidulans | - |
CMI 38,595 S eta, sulfateless mutant | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.8.2.6 | partial | Aspergillus nidulans |
EC Number | Storage Stability | Organism |
---|---|---|
2.8.2.6 | -20°C, freezing and storing for 3 days results in complete loss of activity | Aspergillus nidulans |
2.8.2.6 | 2°C, more stable after (NH4)2SO4 or acetone fractionation, activity is reduced by 85% during 4 weeks | Aspergillus nidulans |
2.8.2.6 | 2°C, very unstable, loses 95% of its activity after 5 days | Aspergillus nidulans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.2.6 | 3'-phosphoadenylylsulfate + choline | - |
Aspergillus nidulans | adenosine 3',5'-bisphosphate + choline sulfate | - |
ir | |
2.8.2.6 | 3'-phosphoadenylylsulfate + choline | sulfate transfer appears to be irreversible in vitro | Aspergillus nidulans | adenosine 3',5'-bisphosphate + choline sulfate | - |
ir | |
2.8.2.6 | 3'-phosphoadenylylsulfate + N,N-dimethylaminoethanol | - |
Aspergillus nidulans | adenosine 3',5'-bisphosphate + N,N-dimethylaminoethanol O-sulfate | - |
? | |
2.8.2.6 | 3'-phosphoadenylylsulfate + N,N-dimethylethylaminoethanol | - |
Aspergillus nidulans | adenosine 3',5'-bisphosphate + N,N-dimethylethylaminoethanol O-sulfate | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.8.2.6 | 20 | 30 | - |
Aspergillus nidulans |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.8.2.6 | 46 | - |
no loss of activity during 10 min at pH 9.2, 30% loss at pH 7.3, 34% loss at pH 5.0 | Aspergillus nidulans |
2.8.2.6 | 55 | - |
almost completely inactivated | Aspergillus nidulans |
2.8.2.6 | 58.5 | - |
complete loss of activity at pH 5.0, 85% activity retained at pH 9.2 | Aspergillus nidulans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.8.2.6 | 7.8 | - |
- |
Aspergillus nidulans |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.8.2.6 | 5 | 10 | about 50% of activity maximum at pH 5.8 and 10.1 | Aspergillus nidulans |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.8.2.6 | 4.5 | 12 | stable at room temperature in the pH range 8.0-11.0, above pH 11.0 enzyme is rapidly inactivated, 80% inactivation at pH 12.0, below pH 8.0 stablity declines slowly with decreasing pH, maximum stability at pH 8,5-10.5 | Aspergillus nidulans |