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Literature summary extracted from

  • Ohisalo, J.J.; Andersson, S.M.; Pispa, J.P.
    Partial purification and properties of frog liver tyrosine aminotransferase (1977), Biochem. J., 163, 411-417.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.6.1.5 adrenaline activation is independent of the concentration of tyrosine, maximal activation achieved with 7-10 micromolar of adrenaline Rana temporaria
2.6.1.5 dopamine slight activation Rana temporaria
2.6.1.5 metanephrine slight activation Rana temporaria
2.6.1.5 noradrenaline slight activation Rana temporaria

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.5 Aminooxyacetate probably acts reacting with the cofactor Rana temporaria
2.6.1.5 Canaline probably acts reacting with the cofactor Rana temporaria
2.6.1.5 homogentisate half-maximal inhibition with 15 micromolar Rana temporaria

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.5 additional information
-
 additional information non-Michaelis complex kinetic Rana temporaria

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.5 85000
-
 gel filtration, isoforms I and II Rana temporaria

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.5 L-tyrosine + 2-oxoglutarate Rana temporaria
-
 4-hydroxyphenylpyruvate + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.5 Rana temporaria
-
 frog
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.5 partial purification of two isoenzymes, using several chromatographic steps Rana temporaria

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.5 liver
-
 Rana temporaria
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.5 203
-
 isoenzyme I Rana temporaria

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.5 L-tyrosine + 2-oxoglutarate
-
 Rana temporaria 4-hydroxyphenylpyruvate + L-glutamate
-
 ?
2.6.1.5 L-tyrosine + 2-oxoglutarate specific for 2-oxoglutarate as the amino group acceptor Rana temporaria 4-hydroxyphenylpyruvate + L-glutamate
-
 ?
2.6.1.5 L-tyrosine + oxaloacetate no activity Rana temporaria 4-hydroxyphenylpyruvate + L-aspartate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.5 37
-
 assay at Rana temporaria