EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.69 | EDTA | 1 mM, increase in activity | Streptomyces cattleya |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.69 | Chloroacetaldehyde | 0.25 mM, time-dependent inactivation | Streptomyces cattleya | |
1.2.1.69 | Cu2+ | 1 mM, strong inhibition | Streptomyces cattleya | |
1.2.1.69 | iodoacetamide | 0.05 mM, strong inhibition | Streptomyces cattleya | |
1.2.1.69 | Mg2+ | 1 mM, 80% loss of activity, possibly due to a decreased dissociation rate of NADH from the enzyme | Streptomyces cattleya |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.69 | 0.08 | - |
fluoroacetaldehyde | - |
Streptomyces cattleya | |
1.2.1.69 | 0.15 | - |
glycoaldehyde | - |
Streptomyces cattleya | |
1.2.1.69 | 0.32 | - |
benzaldehyde | - |
Streptomyces cattleya | |
1.2.1.69 | 0.81 | - |
acetaldehyde | - |
Streptomyces cattleya |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.1.69 | 55000 | - |
4 * 55000, SDS-PAGE | Streptomyces cattleya |
1.2.1.69 | 200000 | - |
gel filtration | Streptomyces cattleya |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.69 | fluroracetaldehyde + NAD+ + H2O | Streptomyces cattleya | involved in fluorometabolite biosynthesis | fluoroacetate + NADH | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.69 | Streptomyces cattleya | - |
NRRL 8057 | - |
1.2.1.69 | Streptomyces cattleya NRRL 8057 | - |
NRRL 8057 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.1.69 | ammonium sulfate, anion-exchange, 5'-AMP-agarose | Streptomyces cattleya |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.2.1.69 | 0.889 | - |
- |
Streptomyces cattleya |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.69 | acetaldehyde + NAD+ + H2O | 40% of activity with fluoroacetaldehyde | Streptomyces cattleya | acetate + NADH + H+ | - |
? | |
1.2.1.69 | acetaldehyde + NAD+ + H2O | 40% of activity with fluoroacetaldehyde | Streptomyces cattleya NRRL 8057 | acetate + NADH + H+ | - |
? | |
1.2.1.69 | benzaldehyde + NAD+ + H2O | 65% of activity with fluoroacetaldehyde | Streptomyces cattleya | benzoate + NADH + H+ | - |
? | |
1.2.1.69 | benzaldehyde + NAD+ + H2O | 65% of activity with fluoroacetaldehyde | Streptomyces cattleya NRRL 8057 | benzoate + NADH + H+ | - |
? | |
1.2.1.69 | chloroacetaldehyde + NAD+ + H2O | 80% of activity with fluoroacetaldehyde, inactivation upon extended incubation | Streptomyces cattleya | chloroacetate + NADH + H+ | - |
? | |
1.2.1.69 | chloroacetaldehyde + NAD+ + H2O | 80% of activity with fluoroacetaldehyde, inactivation upon extended incubation | Streptomyces cattleya NRRL 8057 | chloroacetate + NADH + H+ | - |
? | |
1.2.1.69 | DL-glyceraldehyde + NAD+ + H2O | 10% of activity with fluoroacetaldehyde | Streptomyces cattleya | DL-glycerate + NADH + H+ | - |
? | |
1.2.1.69 | fluroracetaldehyde + NAD+ + H2O | - |
Streptomyces cattleya | fluoroacetate + NADH + H+ | - |
? | |
1.2.1.69 | fluroracetaldehyde + NAD+ + H2O | - |
Streptomyces cattleya NRRL 8057 | fluoroacetate + NADH + H+ | - |
? | |
1.2.1.69 | fluroracetaldehyde + NAD+ + H2O | involved in fluorometabolite biosynthesis | Streptomyces cattleya | fluoroacetate + NADH | - |
? | |
1.2.1.69 | glycoaldehyde + NAD+ + H2O | 150% of activity with fluoroacetaldehyde | Streptomyces cattleya | glycolate + NADH + H+ | - |
? | |
1.2.1.69 | propionaldehyde + NAD+ + H2O | 28% of activity with fluoroacetaldehyde | Streptomyces cattleya | propionate + NADH + H+ | - |
? | |
1.2.1.69 | propionaldehyde + NAD+ + H2O | 28% of activity with fluoroacetaldehyde | Streptomyces cattleya NRRL 8057 | propionate + NADH + H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.1.69 | tetramer | 4 * 55000, SDS-PAGE | Streptomyces cattleya |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.69 | 9 | - |
- |
Streptomyces cattleya |