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Literature summary extracted from

  • Murphy, C.D.; Moss, S.J.; O'Hagan, D.
    Isolation of an aldehyde dehydrogenase involved in the oxidation of fluoroacetaldehyde to fluoroacetate in Streptomyces cattleya (2001), Appl. Environ. Microbiol., 67, 4919-4921.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.69 EDTA 1 mM, increase in activity Streptomyces cattleya

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.69 Chloroacetaldehyde 0.25 mM, time-dependent inactivation Streptomyces cattleya
1.2.1.69 Cu2+ 1 mM, strong inhibition Streptomyces cattleya
1.2.1.69 iodoacetamide 0.05 mM, strong inhibition Streptomyces cattleya
1.2.1.69 Mg2+ 1 mM, 80% loss of activity, possibly due to a decreased dissociation rate of NADH from the enzyme Streptomyces cattleya

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.69 0.08
-
fluoroacetaldehyde
-
Streptomyces cattleya
1.2.1.69 0.15
-
glycoaldehyde
-
Streptomyces cattleya
1.2.1.69 0.32
-
benzaldehyde
-
Streptomyces cattleya
1.2.1.69 0.81
-
acetaldehyde
-
Streptomyces cattleya

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.69 55000
-
4 * 55000, SDS-PAGE Streptomyces cattleya
1.2.1.69 200000
-
gel filtration Streptomyces cattleya

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.69 fluroracetaldehyde + NAD+ + H2O Streptomyces cattleya involved in fluorometabolite biosynthesis fluoroacetate + NADH
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.69 Streptomyces cattleya
-
NRRL 8057
-
1.2.1.69 Streptomyces cattleya NRRL 8057
-
NRRL 8057
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.69 ammonium sulfate, anion-exchange, 5'-AMP-agarose Streptomyces cattleya

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.69 0.889
-
-
Streptomyces cattleya

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.69 acetaldehyde + NAD+ + H2O 40% of activity with fluoroacetaldehyde Streptomyces cattleya acetate + NADH + H+
-
?
1.2.1.69 acetaldehyde + NAD+ + H2O 40% of activity with fluoroacetaldehyde Streptomyces cattleya NRRL 8057 acetate + NADH + H+
-
?
1.2.1.69 benzaldehyde + NAD+ + H2O 65% of activity with fluoroacetaldehyde Streptomyces cattleya benzoate + NADH + H+
-
?
1.2.1.69 benzaldehyde + NAD+ + H2O 65% of activity with fluoroacetaldehyde Streptomyces cattleya NRRL 8057 benzoate + NADH + H+
-
?
1.2.1.69 chloroacetaldehyde + NAD+ + H2O 80% of activity with fluoroacetaldehyde, inactivation upon extended incubation Streptomyces cattleya chloroacetate + NADH + H+
-
?
1.2.1.69 chloroacetaldehyde + NAD+ + H2O 80% of activity with fluoroacetaldehyde, inactivation upon extended incubation Streptomyces cattleya NRRL 8057 chloroacetate + NADH + H+
-
?
1.2.1.69 DL-glyceraldehyde + NAD+ + H2O 10% of activity with fluoroacetaldehyde Streptomyces cattleya DL-glycerate + NADH + H+
-
?
1.2.1.69 fluroracetaldehyde + NAD+ + H2O
-
Streptomyces cattleya fluoroacetate + NADH + H+
-
?
1.2.1.69 fluroracetaldehyde + NAD+ + H2O
-
Streptomyces cattleya NRRL 8057 fluoroacetate + NADH + H+
-
?
1.2.1.69 fluroracetaldehyde + NAD+ + H2O involved in fluorometabolite biosynthesis Streptomyces cattleya fluoroacetate + NADH
-
?
1.2.1.69 glycoaldehyde + NAD+ + H2O 150% of activity with fluoroacetaldehyde Streptomyces cattleya glycolate + NADH + H+
-
?
1.2.1.69 propionaldehyde + NAD+ + H2O 28% of activity with fluoroacetaldehyde Streptomyces cattleya propionate + NADH + H+
-
?
1.2.1.69 propionaldehyde + NAD+ + H2O 28% of activity with fluoroacetaldehyde Streptomyces cattleya NRRL 8057 propionate + NADH + H+
-
?

Subunits

EC Number Subunits Comment Organism
1.2.1.69 tetramer 4 * 55000, SDS-PAGE Streptomyces cattleya

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.69 9
-
-
Streptomyces cattleya