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Literature summary extracted from

  • Yoshimoto, T.; Yamamoto, K.; Tsuru, D.
    Extracellular tyrosinase from Streptomyces sp. KY-453: purification and some enzymatic properties (1985), J. Biochem., 97, 1747-1754.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.1 5-hydroxy-2-(hydroxymethyl)-2H-pyran-4-one trivial name kojic acid, 1 mM, complete inhibition of L-dopa oxidation, 94% inhibition of tyrosinase activity Streptomyces sp.
1.14.18.1 diethyldithiocarbamate 1 mM, complete inhibition of L-dopa oxidation, 21% inhibition of tyrosinase activity Streptomyces sp.
1.14.18.1 Hg2+ inhibition of tyrosine hydroxylation Streptomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.1 0.69
-
 phenol
-
 Streptomyces sp.
1.14.18.1 1.11
-
 pyrogallol
-
 Streptomyces sp.
1.14.18.1 3
-
 tyrosine
-
 Streptomyces sp.
1.14.18.1 4
-
 Dopa
-
 Streptomyces sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.18.1 extracellular less than 3% intracellular activity Streptomyces sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.18.1 copper 1 gatom per mol Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.18.1 29000 36000 gel filtration, SDS-PAGE Streptomyces sp.
1.14.18.1 36000
-
 gel filtration Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Streptomyces sp.
-
 KY-453, isolated from soil
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.1 ammonium sulfate, CM-cellulose, Sephadex G-100 Streptomyces sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.18.1 215
-
-
 Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 dopa + 1/2 O2
-
 Streptomyces sp. dopaquinone + H2O
-
 ?
1.14.18.1 L-tyrosine + O2 + AH2
-
 Streptomyces sp. L-dopa + H2O + A
-
 ?
1.14.18.1 phenol + O2 + AH2
-
 Streptomyces sp. o-dihydroxybenzene + H2O + A
-
 ?
1.14.18.1 phenol + O2 + AH2
-
 Streptomyces sp. catechol + H2O + A
-
 ?
1.14.18.1 pyrogallol + O2
-
 Streptomyces sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.18.1 monomer 1 * 29000-36000, SDS-PAGE Streptomyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.18.1 35
-
 with L-dopa Streptomyces sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.18.1 3.2
-
 phenol
-
 Streptomyces sp.
1.14.18.1 7.2
-
 L-tyrosine
-
 Streptomyces sp.
1.14.18.1 9.7
-
 pyrogallol
-
 Streptomyces sp.
1.14.18.1 118
-
 L-Dopa
-
 Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.18.1 6.8
-
 with L-dopa Streptomyces sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.14.18.1 6 9.5 80% activity after 1 h incubation Streptomyces sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.18.1 0.00034
-
 diethyldithiocarbamate non-competitive vs. L-dopa Streptomyces sp.
1.14.18.1 0.0062
-
 diethyldithiocarbamate non-competitive vs. L-tyrosine Streptomyces sp.
1.14.18.1 0.013
-
 5-hydroxy-2-(hydroxymethyl)-2H-pyran-4-one non-competitive vs. L-dopa Streptomyces sp.
1.14.18.1 0.057
-
 5-hydroxy-2-(hydroxymethyl)-2H-pyran-4-one non-competitive vs. L-tyrosine Streptomyces sp.