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Literature summary extracted from

  • Frenkel, E.P.; Kitchens, R.L.
    Purification and properties of acetyl coenzyme A synthetase from baker¦s yeast (1977), J. Biol. Chem., 252, 504-507.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.2.1.1 ATP and AMP stabilize Saccharomyces cerevisiae
6.2.1.1 freezing and thawing causes loss of activity Saccharomyces cerevisiae
6.2.1.1 storage stability is enhanced by high salt and protein concentration Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.1 0.035
-
 CoA
-
 Saccharomyces cerevisiae
6.2.1.1 0.28
-
 acetate
-
 Saccharomyces cerevisiae
6.2.1.1 1.2
-
 ATP
-
 Saccharomyces cerevisiae
6.2.1.1 10
-
 propanoate
-
 Saccharomyces cerevisiae
6.2.1.1 17
-
 Acrylate
-
 Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.1 Mg2+ required Saccharomyces cerevisiae
6.2.1.1 Mg2+ Km: 4.0 mM Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.1 78000
-
 x * 78000, SDS-PAGE Saccharomyces cerevisiae
6.2.1.1 151000
-
 sedimentation equilibrium analysis Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.1 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.1
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.1 additional information
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.1 ATP + acetate + CoA
-
 Saccharomyces cerevisiae AMP + diphosphate + acetyl-CoA
-
 ?
6.2.1.1 ATP + acrylate + CoA
-
 Saccharomyces cerevisiae AMP + diphosphate + acryloyl-CoA
-
 ?
6.2.1.1 ATP + propanoate + CoA
-
 Saccharomyces cerevisiae AMP + diphosphate + propanoyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.1 ? x * 78000, SDS-PAGE Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.1 7.6
-
-
 Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.2.1.1 6.8 8.8 about 50% of maximal activity at pH 6.8 and 8.8 Saccharomyces cerevisiae
6.2.1.1 7.3 8.1 90% of maximal activity at pH 7.3 and 8.1 Saccharomyces cerevisiae