Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Foerster, S.; Roos, J.; Effenberger, F.; Wajant, H.; Sprauer, A.
    The first recombinent hydroxynitrile lyase in the synthesis of (S)-cyanohydrins (1996), Angew. Chem. Int. Ed. Engl., 35, 437-439.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.47 expression in Escherichia coli Manihot esculenta

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.47 Manihot esculenta P52705
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.47 recombinant enzyme Manihot esculenta

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.2.47 leaf
-
 Manihot esculenta
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.47 cyanide + (2E)-but-2-enal
-
 Manihot esculenta (3S,3E)-2-hydroxypent-3-enenitrile 92% enantiomeric excess ?
4.1.2.47 cyanide + (2E)-hex-2-enal
-
 Manihot esculenta (2S,3E)-2-hydroxyhept-3-enenitrile 97% enantiomeric excess ?
4.1.2.47 cyanide + 1,3-benzodioxole-5-carbaldehyde
-
 Manihot esculenta (2S)-1,3-benzodioxol-5-yl(hydroxy)acetonitrile 86% enantiomeric excess ?
4.1.2.47 cyanide + 2,2-dimethylpropanal
-
 Manihot esculenta (2S)-2-hydroxy-3,3-dimethylbutanenitrile 94% enantiomeric excess ?
4.1.2.47 cyanide + 2-chlorobenzaldehyde
-
 Manihot esculenta (2S)-(2-chlorophenyl)(hydroxy)acetonitrile 92% enantiomeric excess ?
4.1.2.47 cyanide + 2-methylpropanal
-
 Manihot esculenta (2S)-2-hydroxy-3-methylbutanenitrile 95% enantiomeric excess ?
4.1.2.47 cyanide + 3,3-dimethylbutan-2-one
-
 Manihot esculenta (2S)-2-hydroxy-2,3,3-trimethylbutanenitrile 78% enantiomeric excess ?
4.1.2.47 cyanide + 4-methoxybenzaldehyde
-
 Manihot esculenta (2S)-hydroxy(4-methoxyphenyl)acetonitrile 98% enantiomeric excess ?
4.1.2.47 cyanide + 4-methylpentan-2-one
-
 Manihot esculenta (2S)-2-hydroxy-2,4-dimethylpentanenitrile 28% enantiomeric excess ?
4.1.2.47 cyanide + benzaldehyde
-
 Manihot esculenta (S)-mandelonitrile 98% enantiomeric excess ?
4.1.2.47 cyanide + butan-2-one
-
 Manihot esculenta (2S)-2-hydroxy-2-methylbutanenitrile 18% enantiomeric excess ?
4.1.2.47 cyanide + cyclohexanecarbaldehyde
-
 Manihot esculenta (2S)-cyclohexyl(hydroxy)acetonitrile 92% enantiomeric excess ?
4.1.2.47 cyanide + heptan-2-one
-
 Manihot esculenta (2S)-2-hydroxy-2-methylheptanenitrile 92% enantiomeric excess ?
4.1.2.47 cyanide + hexan-2-one
-
 Manihot esculenta (2S)-2-hydroxy-2-methylhexanenitrile 80% enantiomeric excess ?
4.1.2.47 cyanide + pentan-2-one
-
 Manihot esculenta (2S)-2-hydroxy-2-methylpentanenitrile 69% enantiomeric excess ?
4.1.2.47 cyanide + pentanal
-
 Manihot esculenta (2S)-2-hydroxyhexanenitrile 91% enantiomeric excess ?
4.1.2.47 cyanide + prop-2-enal
-
 Manihot esculenta (2S)-2-hydroxybut-3-enenitrile 47% enantiomeric excess ?
4.1.2.47 cyanide + propanal
-
 Manihot esculenta (2S)-2-hydroxybutanenitrile 91% enantiomeric excess ?
4.1.2.47 furan-3-carbaldehyde + HCN
-
 Manihot esculenta (2S)-hydroxy(furan-3-yl)ethanenitrile 92% enantiomeric excess ?
4.1.2.47 additional information the enzyme catalyzes enantioselective addition of HCN to aromatic, heteroaromatic and aliphatic aldehydes and ketones forming (S)-aldehyde cyanohydrins or (S)-ketone cyanohydrins Manihot esculenta ?
-
 ?
4.1.2.47 thiophene-2-carbaldehyde + HCN
-
 Manihot esculenta (2S)-hydroxy(thiophen-2-yl)ethanenitrile 96% enantiomeric excess ?
4.1.2.47 thiophene-3-carbaldehyde + HCN
-
 Manihot esculenta (2S)-hydroxy(thiophen-3-yl)ethanenitrile 98% enantiomeric excess ?

Synonyms

EC Number Synonyms Comment Organism
4.1.2.47 (S)-Hydroxynitrile lyase
-
 Manihot esculenta
4.1.2.47 MeHNL
-
 Manihot esculenta