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Literature summary extracted from
- Solubilization, partial purification, and immunodetection of squalene synthetase from tobacco cell suspension cultures (1992), Plant Physiol., 98, 215-220.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.5.1.21 | 0.0095 | - |
farnesyl diphosphate | - |
Nicotiana tabacum |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 2.5.1.21 | microsome | integral membrane protein | Nicotiana tabacum | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.5.1.21 | 47000 | - |
x * 47000, immunoblot analysis of enzyme separated by SDS-PAGE | Nicotiana tabacum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.5.1.21 | farnesyl diphosphate + farnesyl diphosphate | Nicotiana tabacum | first pathway-specific enzyme in cholesterol biosynthesis | diphosphate + presqualene diphosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.5.1.21 | Nicotiana tabacum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.5.1.21 | partial | Nicotiana tabacum |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 2.5.1.21 | cell suspension culture | - |
Nicotiana tabacum | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.5.1.21 | 0.164 | - |
- |
Nicotiana tabacum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.5.1.21 | farnesyl diphosphate + farnesyl diphosphate | first pathway-specific enzyme in cholesterol biosynthesis | Nicotiana tabacum | diphosphate + presqualene diphosphate | - |
? | |
| 2.5.1.21 | farnesyl diphosphate + NAD(P)H | - |
Nicotiana tabacum | squalene + diphosphate + NAD(P)+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.5.1.21 | ? | x * 47000, immunoblot analysis of enzyme separated by SDS-PAGE | Nicotiana tabacum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.5.1.21 | 35 | - |
assay at | Nicotiana tabacum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.5.1.21 | 6 | - |
- |
Nicotiana tabacum |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.5.1.21 | NADH | preference for NADPH over NADH | Nicotiana tabacum | |
| 2.5.1.21 | NADPH | preference for NADPH over NADH | Nicotiana tabacum | |
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