EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.1 | 1.18 | - |
Inosine | - |
Geobacillus stearothermophilus | |
2.4.2.1 | 1.29 | - |
guanosine | - |
Geobacillus stearothermophilus | |
2.4.2.2 | 1.38 | - |
uridine | - |
Haemophilus influenzae | |
2.4.2.2 | 1.9 | - |
thymidine | - |
Haemophilus influenzae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.2.1 | 30000 | - |
3 * 30000, SDS-PAGE | Geobacillus stearothermophilus |
2.4.2.1 | 90000 | - |
gel filtration | Geobacillus stearothermophilus |
2.4.2.2 | 46000 | - |
2 * 46000, SDS-PAGE | Haemophilus influenzae |
2.4.2.2 | 92000 | - |
gel filtration | Haemophilus influenzae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.2.1 | Geobacillus stearothermophilus | - |
TH 6-2 | - |
2.4.2.1 | Geobacillus stearothermophilus TH 6-2 | - |
TH 6-2 | - |
2.4.2.2 | Haemophilus influenzae | - |
TH 6-2 | - |
2.4.2.2 | Haemophilus influenzae TH 6-2 | - |
TH 6-2 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.2.1 | - |
Geobacillus stearothermophilus |
2.4.2.2 | - |
Haemophilus influenzae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.2.1 | 91.6 | - |
- |
Geobacillus stearothermophilus |
2.4.2.2 | 205 | - |
- |
Haemophilus influenzae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.1 | adenosine + phosphate | no activity | Geobacillus stearothermophilus | adenine + alpha-D-ribose 1-phosphate | - |
? | |
2.4.2.1 | adenosine + phosphate | no activity | Geobacillus stearothermophilus TH 6-2 | adenine + alpha-D-ribose 1-phosphate | - |
? | |
2.4.2.1 | deoxyguanosine + phosphate | 85% of the activity with inosine | Geobacillus stearothermophilus | guanine + alpha-D-deoxyribose 1-phosphate | - |
? | |
2.4.2.1 | deoxyguanosine + phosphate | 85% of the activity with inosine | Geobacillus stearothermophilus TH 6-2 | guanine + alpha-D-deoxyribose 1-phosphate | - |
? | |
2.4.2.1 | deoxyinosine + phosphate | as active as inosine | Geobacillus stearothermophilus | hypoxanthine + alpha-D-deoxyribose 1-phosphate | - |
r | |
2.4.2.1 | deoxyinosine + phosphate | as active as inosine | Geobacillus stearothermophilus TH 6-2 | hypoxanthine + alpha-D-deoxyribose 1-phosphate | - |
r | |
2.4.2.1 | guanosine + phosphate | 84% of the activity with guanosine | Geobacillus stearothermophilus | guanine + alpha-D-ribose 1-phosphate | - |
? | |
2.4.2.1 | guanosine + phosphate | 84% of the activity with guanosine | Geobacillus stearothermophilus TH 6-2 | guanine + alpha-D-ribose 1-phosphate | - |
? | |
2.4.2.1 | inosine + phosphate | - |
Geobacillus stearothermophilus | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
2.4.2.1 | inosine + phosphate | - |
Geobacillus stearothermophilus TH 6-2 | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
2.4.2.1 | xanthosine + phosphate | - |
Geobacillus stearothermophilus | alpha-D-ribose 1-phosphate + xanthine | - |
? | |
2.4.2.2 | deoxyuridine + phosphate | 147% of the activity with uridine | Haemophilus influenzae | uracil + 2-deoxy-D-ribose 1-phosphate | - |
? | |
2.4.2.2 | deoxyuridine + phosphate | 147% of the activity with uridine | Haemophilus influenzae TH 6-2 | uracil + 2-deoxy-D-ribose 1-phosphate | - |
? | |
2.4.2.2 | ribofuranosyl thymine + phosphate | - |
Haemophilus influenzae | thymine + alpha-D-ribose 1-phosphate | - |
? | |
2.4.2.2 | ribofuranosyl thymine + phosphate | - |
Haemophilus influenzae TH 6-2 | thymine + alpha-D-ribose 1-phosphate | - |
? | |
2.4.2.2 | thymidine + phosphate | 99% of the activity with uridine | Haemophilus influenzae | thymine + 2-deoxy-D-ribose 1-phosphate | - |
? | |
2.4.2.2 | thymidine + phosphate | 99% of the activity with uridine | Haemophilus influenzae TH 6-2 | thymine + 2-deoxy-D-ribose 1-phosphate | - |
? | |
2.4.2.2 | uridine + phosphate | - |
Haemophilus influenzae | uracil + alpha-D-ribose 1-phosphate | - |
? | |
2.4.2.2 | uridine + phosphate | - |
Haemophilus influenzae TH 6-2 | uracil + alpha-D-ribose 1-phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.2.1 | trimer | 3 * 30000, SDS-PAGE | Geobacillus stearothermophilus |
2.4.2.2 | dimer | 2 * 46000, SDS-PAGE | Haemophilus influenzae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.2.1 | 70 | - |
- |
Geobacillus stearothermophilus |
2.4.2.2 | 70 | - |
- |
Haemophilus influenzae |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.2.1 | 70 | - |
stable below | Geobacillus stearothermophilus |
2.4.2.2 | 60 | - |
stable below | Haemophilus influenzae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.2.1 | 8 | - |
- |
Geobacillus stearothermophilus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.4.2.1 | 5 | 9 | stable from pH 5.0 to pH 9.0 | Geobacillus stearothermophilus |
2.4.2.2 | 5 | 9 | stable | Haemophilus influenzae |