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Literature summary extracted from

  • Koszalka, G.W.; Vanhooke, J.; Short, S.A.; Hall, W.W.
    Purification and properties of inosine-guanosine phosphorylase from Escherichia coli K-12 (1988), J. Bacteriol., 170, 3493-3498.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.1 adenosine
-
 Escherichia coli
2.4.2.1 deoxyadenosine
-
 Escherichia coli
2.4.2.1 hypoxanthine arabinoside
-
 Escherichia coli
2.4.2.1 PCMB dithiothreitol restores activity Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.1 0.0033
-
 hypoxanthine
-
 Escherichia coli
2.4.2.1 0.0041
-
 guanosine
-
 Escherichia coli
2.4.2.1 0.044
-
 deoxyguanosine
-
 Escherichia coli
2.4.2.1 0.051
-
 Xanthosine
-
 Escherichia coli
2.4.2.1 0.059
-
 alpha-D-ribose 1-phosphate
-
 Escherichia coli
2.4.2.1 0.062
-
 2'-deoxyinosine
-
 Escherichia coli
2.4.2.1 0.11
-
 guanosine
-
 Escherichia coli
2.4.2.1 0.34
-
 Inosine
-
 Escherichia coli
2.4.2.1 0.6
-
 2'-deoxyinosine
-
 Escherichia coli
2.4.2.1 0.76
-
 phosphate
-
 Escherichia coli
2.4.2.1 2.6
-
 2',3'-dideoxyinosine
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.1 180000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Escherichia coli
-
 K-12, inosine-guanosine phosphorylase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.1 partial Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.1 0.0141
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 2',3'-dideoxyinosine + phosphate
-
 Escherichia coli hypoxanthine + alpha-D-2,3-dideoxyribose 1-phosphate
-
 ?
2.4.2.1 2'-deoxyinosine + phosphate
-
 Escherichia coli hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 adenosine + phosphate no activity Escherichia coli adenine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 deoxyguanosine + phosphate
-
 Escherichia coli guanine + alpha-D-deoxyribose 1-phosphate
-
 ?
2.4.2.1 deoxyinosine + phosphate 2'-deoxyinosine and 5'-deoxyinosine Escherichia coli hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
 r
2.4.2.1 guanosine + phosphate
-
 Escherichia coli guanine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate
-
 Escherichia coli hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 xanthosine + phosphate
-
 Escherichia coli alpha-D-ribose 1-phosphate + xanthine
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.1 45
-
 half-life at pH 6-7: 8-9 min. Half-life at pH 7-8: 5-7 min. At pH 6.5 in presence of 5 mg/ml bovine serum albumin half-life is 17 min Escherichia coli
2.4.2.1 50
-
 5 min, 75-85% loss of activity, 1 mM hypoxanthine significantly protects against thermal denaturation Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.1 additional information
-
 additional information
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.1 6.6
-
 phosphorolysis Escherichia coli
2.4.2.1 6.8
-
 synthetic direction Escherichia coli