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Literature summary extracted from

  • Jensen, K.F.; Nygaard, P.
    Purine nucleoside phosphorylase from Escherichia coli and Salmonella typhimurium. Purification and some properties (1975), Eur. J. Biochem., 51, 253-265.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.1
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.1
-
 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.1 PCMB
-
 Escherichia coli
2.4.2.1 PCMB
-
 Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.1 0.04
-
 alpha-D-ribose 1-phosphate reaction with adenine Escherichia coli
2.4.2.1 0.07
-
 Inosine
-
 Escherichia coli
2.4.2.1 0.09
-
 Inosine
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.1 0.1
-
 phosphate reaction with inosine Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.1 0.1
-
 deoxyribose 1-phosphate reaction with adenine Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.1 0.1
-
 alpha-D-deoxyribose 1-phosphate reaction with adenine Escherichia coli
2.4.2.1 0.12
-
 phosphate reaction with inosine Escherichia coli
2.4.2.1 0.17
-
 deoxyinosine
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.1 0.18
-
 deoxyinosine
-
 Escherichia coli
2.4.2.1 0.63
-
 arsenate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.1 0.8
-
 arsenate reaction with inosine Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.1 23700
-
 6 * 23700, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.1 23700
-
 6 * 23700, SDS-PAGE Escherichia coli
2.4.2.1 138000
-
 gel filtration Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.1 138000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Escherichia coli
-
-
-
2.4.2.1 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.1
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.1
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.1 195
-
-
 Escherichia coli
2.4.2.1 301
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

EC Number Storage Stability Organism
2.4.2.1 4°C, stable for 2 years Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.1 4°C, stable for 2 years Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 adenine + deoxyribose 1-phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium deoxyadenosine + phosphate
-
 ?
2.4.2.1 adenosine + arsenate
-
 Salmonella enterica subsp. enterica serovar Typhimurium adenine + alpha-D-ribose 1-arsenate
-
 ?
2.4.2.1 adenosine + arsenate
-
 Escherichia coli adenine + alpha-D-ribose 1-arsenate
-
 ?
2.4.2.1 adenosine + phosphate 78% of the activity with deoxyguanosine Salmonella enterica subsp. enterica serovar Typhimurium adenine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 adenosine + phosphate 61% of the activity with deoxyguanosine Escherichia coli adenine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 deoxyadenosine + phosphate 78% of the activity with deoxyguanosine Salmonella enterica subsp. enterica serovar Typhimurium alpha-D-ribose 1-phosphate + adenine
-
 ?
2.4.2.1 deoxyadenosine + phosphate 61% of the activity with deoxyguanosine Escherichia coli alpha-D-ribose 1-phosphate + adenine
-
 ?
2.4.2.1 deoxyguanosine + phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium guanine + alpha-D-deoxyribose 1-phosphate
-
 ?
2.4.2.1 deoxyguanosine + phosphate
-
 Escherichia coli guanine + alpha-D-deoxyribose 1-phosphate
-
 ?
2.4.2.1 deoxyinosine + phosphate 88% of the activity with deoxyguanosine Salmonella enterica subsp. enterica serovar Typhimurium hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
 r
2.4.2.1 deoxyinosine + phosphate 88% of the activity with deoxyguanosine Escherichia coli hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
 r
2.4.2.1 guanosine + arsenate
-
 Salmonella enterica subsp. enterica serovar Typhimurium guanine + alpha-D-ribose 1-arsenate
-
 ?
2.4.2.1 guanosine + arsenate
-
 Escherichia coli guanine + alpha-D-ribose 1-arsenate
-
 ?
2.4.2.1 guanosine + phosphate 81% of the activity with deoxyguanosine Salmonella enterica subsp. enterica serovar Typhimurium guanine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 guanosine + phosphate 48% of the activity with deoxyguanosine Escherichia coli guanine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 inosine + arsenate
-
 Salmonella enterica subsp. enterica serovar Typhimurium hypoxanthine + ribose 1-arsenate
-
 ?
2.4.2.1 inosine + arsenate
-
 Escherichia coli hypoxanthine + ribose 1-arsenate
-
 ?
2.4.2.1 inosine + phosphate 82% of the activity with deoxyguanosine Salmonella enterica subsp. enterica serovar Typhimurium hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate 46% of the activity with deoxyguanosine Escherichia coli hypoxanthine + alpha-D-ribose 1-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.2.1 hexamer 6 * 23700, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.1 hexamer 6 * 23700, SDS-PAGE Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.1 69
-
 0.2 M Tris-succinate buffer, pH 7.1, half-life: 11 min. In 0.2 M potassium phosphate buffer, pH 7.1, with 2 mM deoxyadenosine, half-life: 59 min Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.1 6.8
-
 synthesis of inosine from hypoxanthine Escherichia coli
2.4.2.1 7.1
-
 phosphorolysis of deoxyinosine Escherichia coli
2.4.2.1 7.5
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.1 7.5
-
 phosphorolysis of inosine Escherichia coli