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Literature summary extracted from

  • Lewis, A.S.; Glantz, M.D.
    Monomeric purine nucleoside phosphorylase from rabbit liver. Purification and characterization (1976), J. Biol. Chem., 251, 407-413.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.2.1 freezing of the solubilized enzyme, especially in dilute solutions, causes complete loss of activity Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.1 alpha-D-ribose 1-phosphate
-
 Oryctolagus cuniculus
2.4.2.1 guanine
-
 Oryctolagus cuniculus
2.4.2.1 hypoxanthine
-
 Oryctolagus cuniculus
2.4.2.1 PCMB
-
 Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.1 0.0182
-
 guanine
-
 Oryctolagus cuniculus
2.4.2.1 0.05
-
 Inosine
-
 Oryctolagus cuniculus
2.4.2.1 0.1
-
 deoxyguanosine
-
 Oryctolagus cuniculus
2.4.2.1 0.133
-
 Inosine
-
 Oryctolagus cuniculus
2.4.2.1 0.134
-
 alpha-D-ribose 1-phosphate
-
 Oryctolagus cuniculus
2.4.2.1 15.4
-
 phosphate
-
 Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.1 39000
-
 x * 39000, SDS-PAGE Oryctolagus cuniculus
2.4.2.1 45000
-
 gel filtration Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Oryctolagus cuniculus
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.4.2.1 highly susceptible to photooxidation in presence of methylene blue, maximal photoinactivation near pH 8.5 Oryctolagus cuniculus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.1
-
 Oryctolagus cuniculus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.2.1 purine ribonucleoside + phosphate = purine + alpha-D-ribose 1-phosphate sequential bireactant mechanism Oryctolagus cuniculus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.1 liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.1 47.12
-
-
 Oryctolagus cuniculus

Storage Stability

EC Number Storage Stability Organism
2.4.2.1 4°C, enzyme containing 2-mercaptoethanol prior to precipitation with ammonium sulfate, near pH 7, retains maximal catalytic activity for several months when stored as a precipitate Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 deoxyguanosine + phosphate
-
 Oryctolagus cuniculus guanine + alpha-D-deoxyribose 1-phosphate
-
 ?
2.4.2.1 guanosine + phosphate
-
 Oryctolagus cuniculus guanine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate
-
 Oryctolagus cuniculus hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 xanthosine + phosphate reaction only at high concentrations Oryctolagus cuniculus alpha-D-ribose 1-phosphate + xanthine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.1 ? x * 39000, SDS-PAGE Oryctolagus cuniculus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.1 60
-
 20 min, 10% loss of activity Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.1 6 8
-
 Oryctolagus cuniculus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.1 0.361
-
 alpha-D-ribose 1-phosphate
-
 Oryctolagus cuniculus