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Literature summary extracted from
- Purine nucleoside phosphorylase from rabbit liver (1978), Methods Enzymol., 51, 524-530.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 2.4.2.1 | stabilized by 2-mercaptoethanol during the purification | Oryctolagus cuniculus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.4.2.1 | alpha-D-ribose 1-phosphate | product inhibition | Oryctolagus cuniculus |  |
| 2.4.2.1 | guanine | product inhibition | Oryctolagus cuniculus | |
| 2.4.2.1 | hypoxanthine | product inhibition | Oryctolagus cuniculus | |
| 2.4.2.1 | PCMB | - |
Oryctolagus cuniculus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.4.2.1 | 0.05 | - |
Inosine | - |
Oryctolagus cuniculus | |
| 2.4.2.1 | 0.05 | - |
guanosine | - |
Oryctolagus cuniculus | |
| 2.4.2.1 | 0.1 | - |
deoxyguanosine | - |
Oryctolagus cuniculus | |
| 2.4.2.1 | 0.133 | - |
Inosine | - |
Oryctolagus cuniculus | |
| 2.4.2.1 | 15.4 | - |
phosphate | - |
Oryctolagus cuniculus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.4.2.1 | 30500 | - |
2 * 30500, SDS-PAGE | Oryctolagus cuniculus |
| 2.4.2.1 | 61000 | - |
gel filtration | Oryctolagus cuniculus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.4.2.1 | Oryctolagus cuniculus | - |
- |
- |
Oxidation Stability
| EC Number | Oxidation Stability | Organism |
|---|---|---|
| 2.4.2.1 | highly susceptible to photooxidation in presence of methylene blue, maximal photoinactivation near pH 8.5 | Oryctolagus cuniculus |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.4.2.1 | - |
Oryctolagus cuniculus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 2.4.2.1 | liver | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.4.2.1 | 47.12 | - |
- |
Oryctolagus cuniculus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.2.1 | deoxyguanosine + phosphate | - |
Oryctolagus cuniculus | guanine + alpha-D-deoxyribose 1-phosphate | - |
? | |
| 2.4.2.1 | guanosine + phosphate | - |
Oryctolagus cuniculus | guanine + alpha-D-ribose 1-phosphate | - |
? | |
| 2.4.2.1 | inosine + phosphate | - |
Oryctolagus cuniculus | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.4.2.1 | dimer | 2 * 30500, SDS-PAGE | Oryctolagus cuniculus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.2.1 | 6 | 8 | - |
Oryctolagus cuniculus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.2.1 | 5.5 | 9 | 40°C, 10 min, stable | Oryctolagus cuniculus |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.4.2.1 | 0.00568 | - |
PCMB | - |
Oryctolagus cuniculus | |
| 2.4.2.1 | 0.0125 | - |
guanine | - |
Oryctolagus cuniculus | |
| 2.4.2.1 | 0.025 | - |
hypoxanthine | - |
Oryctolagus cuniculus | |
| 2.4.2.1 | 0.361 | - |
alpha-D-ribose 1-phosphate | - |
Oryctolagus cuniculus | |
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Release 2023.1 (January 2023)
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