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Literature summary extracted from

  • Hori, N.; Watanabe, M.; Yamazaki, Y.; Mikami, Y.
    Purification and characterization of second thermostable purine nucleoside phosphorylase in Bacillus stearothermophilus JTS 859 (1989), Agric. Biol. Chem., 53, 3219-3224.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.1 PCMB no inhibition of PUNPII Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.1 0.075
-
 adenosine
-
 Geobacillus stearothermophilus
2.4.2.1 0.19
-
 2'-deoxyadenosine
-
 Geobacillus stearothermophilus
2.4.2.1 0.22
-
 Inosine
-
 Geobacillus stearothermophilus
2.4.2.1 0.34
-
 guanosine
-
 Geobacillus stearothermophilus
2.4.2.1 0.92
-
 2'-deoxyinosine
-
 Geobacillus stearothermophilus
2.4.2.1 1.9
-
 2'-deoxyguanosine
-
 Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.1 113000
-
 gel filtration Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Geobacillus stearothermophilus
-
 JTS 859
-
2.4.2.1 Geobacillus stearothermophilus JTS 859
-
 JTS 859
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.1 PUNPI and PUNPII Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.1 50.6
-
 PUNPII Geobacillus stearothermophilus
2.4.2.1 405
-
 PUNPI Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 adenosine + phosphate 160% of the activity with inosine Geobacillus stearothermophilus adenine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 adenosine + phosphate activity with OUNOII, no activity with PUNPI Geobacillus stearothermophilus adenine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 adenosine + phosphate 160% of the activity with inosine Geobacillus stearothermophilus JTS 859 adenine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 deoxyadenosine + phosphate 2'-deoxyadenosine, 160% of the activity with inosine Geobacillus stearothermophilus alpha-D-ribose 1-phosphate + adenine
-
 ?
2.4.2.1 deoxyguanosine + phosphate 2'-deoxyguanosine, 469% of the activity with inosine Geobacillus stearothermophilus guanine + alpha-D-deoxyribose 1-phosphate
-
 ?
2.4.2.1 deoxyguanosine + phosphate 2'-deoxyguanosine, 469% of the activity with inosine Geobacillus stearothermophilus JTS 859 guanine + alpha-D-deoxyribose 1-phosphate
-
 ?
2.4.2.1 deoxyinosine + phosphate 2'-deoxyinosine, 691% of the activity with inosine Geobacillus stearothermophilus hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
 r
2.4.2.1 deoxyinosine + phosphate 2'-deoxyinosine, 691% of the activity with inosine Geobacillus stearothermophilus JTS 859 hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
 r
2.4.2.1 guanosine + phosphate 53% of the activity with inosine Geobacillus stearothermophilus guanine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 guanosine + phosphate 53% of the activity with inosine Geobacillus stearothermophilus JTS 859 guanine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 inosine + phosphate
-
 Geobacillus stearothermophilus hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 inosine + phosphate
-
 Geobacillus stearothermophilus JTS 859 hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.1 70
-
-
 Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.1 70
-
 half-life of isoenzyme PUNPII is 15 min Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.1 7 11
-
 Geobacillus stearothermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.2.1 5 11.5 pH 5.0: about 50% of maximal activity, pH 11.5: about 85% of maximal activity Geobacillus stearothermophilus