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Literature summary extracted from

  • Robertson, B.C.; Hoffee, P.A.
    Purification and properties of purine nucleoside phosphorylase from Salmonella typhimurium (1973), J. Biol. Chem., 248, 2040-2043.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.1 0.047
-
 deoxyinosine
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.1 0.05
-
 Inosine
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.1 0.37
-
 phosphate reaction with inosine Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.1 Co2+ 1 mM, 1.65fold stimulation Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.1 23500
-
 6 * 23500, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.1 130000
-
 gel filtration Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.1 141000
-
 equilibrium sedimentation Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Salmonella enterica subsp. enterica serovar Typhimurium
-
 LT-2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.1
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.1 160
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 adenosine + phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium adenine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 deoxyadenosine + phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium alpha-D-ribose 1-phosphate + adenine
-
 ?
2.4.2.1 deoxyguanosine + phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium guanine + alpha-D-deoxyribose 1-phosphate
-
 ?
2.4.2.1 deoxyinosine + phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
 r
2.4.2.1 guanosine + phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium guanine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 inosine + phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.1 hexamer 6 * 23500, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.1 7.5
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium