EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.85 | bovine serum albumin | activates | Sorghum bicolor | |
2.4.1.85 | dithiothreitol | requirement, 5 mM | Sorghum bicolor |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.85 | cloning of a full-length cDNA encoding enzyme, expression in Escherichia coli JM109, 492-amino acids translation product | Sorghum bicolor |
EC Number | General Stability | Organism |
---|---|---|
2.4.1.85 | activity is largely uneffected by both freezing at -80°C and addition of glycerol, DTT stabilizes | Sorghum bicolor |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.85 | 4-hydroxybenzaldehyde | potent inhibitor, 1 mg/ml bovine serum albumin protects | Sorghum bicolor | |
2.4.1.85 | 4-hydroxymandelonitrile | possibly an inhibitor, 1 mg/ml bovine serum albumin protects | Sorghum bicolor | |
2.4.1.85 | benzaldehyde | potent inhibitor, 1 mg/ml bovine serum albumin protects | Sorghum bicolor | |
2.4.1.85 | Dhurrin | potent inhibitor, 1 mg/ml bovine serum albumin protects | Sorghum bicolor | |
2.4.1.85 | mandelonitrile | potent inhibitor, 1 mg/ml bovine serum albumin protects | Sorghum bicolor | |
2.4.1.85 | sambunigrin | potent inhibitor, 1 mg/ml bovine serum albumin protects | Sorghum bicolor |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.4.1.85 | soluble | - |
Sorghum bicolor | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.85 | 52900 | - |
x * 52900, calculated from the amino acid sequence | Sorghum bicolor |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.85 | UDPglucose + (S)-4-hydroxymandelonitrile | Sorghum bicolor | endogenous substrate is p-hydroxymandelonitrile, final step in the biosynthesis of dhurrin, transformation of the labile cyanohydrin into a stable storage form | UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.85 | Sorghum bicolor | Q9SBL1 | Pacific seeds, Australia, cultivar MR31 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.85 | 420fold, native and recombinant enzyme, expressed in Escherichia coli JM109 | Sorghum bicolor |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.1.85 | seedling | etiolated | Sorghum bicolor | - |
EC Number | Storage Stability | Organism |
---|---|---|
2.4.1.85 | 4°C, 2 days, lowering the concentration of DTT from 5 to 2 mM results in a 10fold decrease in activity | Sorghum bicolor |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.85 | additional information | exclusively specific for the presence of a benzyl group in the substrate, no substrate: acetone cyanohydrin, hydroquinone, p-hydroxybenzaldehyde, gentisic acid, caffeic acid, 2-hydroxy cinnamic acid, resveratrol, salicylic acid, p-hydroxymandelic acid, quercetin, caynidin, biochanin A, naringenin, apigenin, indole acetic acid, tomatidine | Sorghum bicolor | additional information | - |
? | |
2.4.1.85 | UDPalpha-D-glucose + benzoic acid | at 4% of the rate with p-hydroxymandelonitrile | Sorghum bicolor | UDP + 1-O-benzoyl-beta-D-glucose | - |
? | |
2.4.1.85 | UDPglucose + (S)-4-hydroxymandelonitrile | C-terminal region encodes the UDPglucose binding domain | Sorghum bicolor | UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside | identical with dhurrin | ? | |
2.4.1.85 | UDPglucose + (S)-4-hydroxymandelonitrile | stereospecific for the S-enantiomer | Sorghum bicolor | UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside | identical with dhurrin | ? | |
2.4.1.85 | UDPglucose + (S)-4-hydroxymandelonitrile | endogenous substrate is p-hydroxymandelonitrile, final step in the biosynthesis of dhurrin, transformation of the labile cyanohydrin into a stable storage form | Sorghum bicolor | UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside | - |
? | |
2.4.1.85 | UDPglucose + (S)-mandelonitrile | at 78% of the rate with p-hydroxymandelonitrile | Sorghum bicolor | UDP + (S)-mandelonitrile beta-D-glucoside | - |
? | |
2.4.1.85 | UDPglucose + benzyl alcohol | at 13% of the rate with p-hydroxymandelonitrile | Sorghum bicolor | UDP + benzylglucoside | - |
? | |
2.4.1.85 | UDPglucose + geraniol | at 11% of the rate with p-hydroxymandelonitrile | Sorghum bicolor | UDP + geraniol glucoside | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.85 | ? | x * 50000-55000, SDS-PAGE | Sorghum bicolor |
2.4.1.85 | ? | x * 52900, calculated from the amino acid sequence | Sorghum bicolor |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.85 | sbHMNGT | - |
Sorghum bicolor |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.85 | 30 | - |
assay at | Sorghum bicolor |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.85 | additional information | - |
theoretical pI of 5.3 | Sorghum bicolor |