EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.69 | G-3634-A | activity is optimal with 5 mg/ml of the cationic detergent | Bos taurus | |
2.4.1.344 | G-3634-A | cationic detergent, optimal rate of reaction at 5 mg/ml | Bos taurus | |
2.4.1.344 | taurocholate | about 36% as effective as G-3634-A | Bos taurus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.69 | EDTA | 5 mM, 95% loss of activity | Bos taurus | |
2.4.1.344 | EDTA | 5 mM, 5% residual activtiy | Bos taurus | |
2.4.1.344 | GTP | 0.5 mM, 30% inhibition | Bos taurus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.69 | 0.36 | - |
GDP-L-fucose | - |
Bos taurus | |
2.4.1.69 | 0.6 | - |
Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc-ceramide | - |
Bos taurus | |
2.4.1.69 | 1.5 | - |
Galalpha(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc-ceramide | - |
Bos taurus | |
2.4.1.344 | 0.36 | - |
beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-glucosylceramide | pH 6.4, 37°C | Bos taurus | |
2.4.1.344 | 0.6 | - |
GDP-beta-L-fucose | pH 6.4, 37°C | Bos taurus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.4.1.69 | membrane | - |
Bos taurus | 16020 | - |
2.4.1.344 | endoplasmic reticulum | - |
Bos taurus | 5783 | - |
2.4.1.344 | membrane | - |
Bos taurus | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.69 | Ca2+ | stimulates dialyzed enzyme | Bos taurus | |
2.4.1.69 | Mg2+ | partially prevents inactivation in presence of EDTA | Bos taurus | |
2.4.1.69 | Mn2+ | partially prevents inactivation in presence of EDTA | Bos taurus | |
2.4.1.69 | Zn2+ | stimulates dialyzed enzyme | Bos taurus | |
2.4.1.344 | Mg2+ | 50-70% recovery of activity after EDTA-treatment | Bos taurus | |
2.4.1.344 | Mn2+ | 50-70% recovery of activity after EDTA-treatment | Bos taurus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.69 | Bos taurus | - |
- |
- |
2.4.1.344 | Bos taurus | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.1.69 | spleen | - |
Bos taurus | - |
2.4.1.344 | spleen | - |
Bos taurus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.69 | GDP-L-fucose + beta-D-galactosyl-R | - |
Bos taurus | GDP + alpha-L-fucosyl-1,2-beta-D-galactosyl-R | - |
? | |
2.4.1.69 | GDP-L-fucose + Galalpha(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc-ceramide | - |
Bos taurus | GDP + L-Fucalpha(1-2)Galalpha(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc-ceramide | - |
? | |
2.4.1.69 | GDP-L-fucose + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc-ceramide | - |
Bos taurus | GDP + L-Fucalpha(1-2)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc-ceramide | - |
? | |
2.4.1.344 | GDP-beta-L-fucose + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-glucosylceramide | - |
Bos taurus | GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-glucosylceramide | - |
? | |
2.4.1.344 | additional information | enzyme catalyzes the transfer of L-fucose from GDP-L-fucose to a tetraglycosylceramide to form the blood group H-related glycosphingolipid | Bos taurus | ? | - |
? | |
2.4.1.344 | additional information | no substrates: glucosamine, UDP-N-acetyl-galactosamine, and CMP-sialic acid | Bos taurus | ? | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.69 | 6.4 | - |
cacodylate-HCl buffer | Bos taurus |
2.4.1.344 | 6.4 | - |
cacodylate buffer | Bos taurus |