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Literature summary extracted from

  • Seely, M.K.; Conn, E.E.
    Hydroxynitrile lyase (Sorghum vulgare) (1971), Methods Enzymol., 17B, 239-244.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.11 0.7
-
D,L-hydroxymandelonitrile
-
Sorghum bicolor
4.1.2.11 0.73
-
D,L-vanillin cyanohydrin
-
Sorghum bicolor
4.1.2.11 0.79
-
DL-mandelonitrile
-
Sorghum bicolor
4.1.2.11 1.2
-
D,L-isovanillin cyanohydrin
-
Sorghum bicolor

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.11 Sorghum bicolor
-
i.e. Sorghum vulgare
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.11
-
Sorghum bicolor

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.2.11 seedling
-
Sorghum bicolor
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.11 188
-
4-hydroxymandelonitrile Sorghum bicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.11 (S)-4-Hydroxymandelonitrile
-
Sorghum bicolor Cyanide + 4-hydroxybenzaldehyde
-
?
4.1.2.11 (S)-mandelonitrile
-
Sorghum bicolor cyanide + benzaldehyde
-
?
4.1.2.11 4-Hydroxymandelonitrile
-
Sorghum bicolor ?
-
?
4.1.2.11 DL-mandelonitrile
-
Sorghum bicolor cyanide + benzaldehyde
-
?
4.1.2.11 isovanillin cyanohydrin
-
Sorghum bicolor cyanide + isovanillin
-
?
4.1.2.11 vanillin cyanohydrin
-
Sorghum bicolor cyanide + vanillin
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.11 5 6
-
Sorghum bicolor