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Literature summary extracted from

  • Schmid, G.; Grisebach, H.
    Enzymic synthesis of lignin precursors. Purification and properties of UDP glucose: coniferyl-alcohol glucosyltransferase from cambial sap of spruce (Picea abies L.) (1982), Eur. J. Biochem., 123, 363-370.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.1.111 addition of high molarity buffer, 200 mM Tris/HCl, or 2-mercaptoethanol, optimal concentration 42 mM, increases stability considerably Picea abies
2.4.1.111 in dilute solutions addition of 10-20% glycerol or ethylene glycol increases stability Picea abies

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.111 coniferin non-competitive, product inhibition Picea abies
2.4.1.111 EDTA no inhibition Picea abies
2.4.1.111 PCMB 0.1 mM, complete inhibition Picea abies
2.4.1.111 UDP non-competitive, product inhibition Picea abies

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.111 0.22
-
 UDPglucose
-
 Picea abies
2.4.1.111 0.25
-
 coniferyl alcohol
-
 Picea abies
2.4.1.111 0.58
-
 sinapyl alcohol
-
 Picea abies
2.4.1.111 0.64
-
 Vanillin
-
 Picea abies

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.111 44000
-
 gel filtration Picea abies
2.4.1.111 50000
-
 1 * 50000, SDS-PAGE Picea abies

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.111 UDPglucose + coniferyl alcohol Picea abies participates in lignification of spruce ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.111 Picea abies
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.111
-
 Picea abies

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.111 UDP-glucose + coniferyl alcohol = UDP + coniferin mechanism Picea abies
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.111 cambium
-
 Picea abies
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.111 2.436
-
-
 Picea abies

Storage Stability

EC Number Storage Stability Organism
2.4.1.111 -20°C, 200 mM Tris/HCl, pH 7.5, 15% glycerol, stable for several months Picea abies
2.4.1.111 0-2°C, several weeks Picea abies
2.4.1.111 glycerol, 15%, stabilizes during storage Picea abies

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.111 additional information TDPglucose can replace UDPglucose with 17% efficiency Picea abies ?
-
 ?
2.4.1.111 UDP-glucose + coniferyl alcohol forward reaction is about six times faster than the reverse reaction Picea abies UDP + coniferin
-
 r
2.4.1.111 UDP-glucose + coniferyl aldehyde glucosylated at 63% the rate of coniferyl alcohol Picea abies UDP + coniferaldehyde-4-O-beta-D-glucopyranoside
-
 ?
2.4.1.111 UDP-glucose + sinapaldehyde glucosylated at 48% the rate of coniferyl alcohol Picea abies UDP + sinapaldehyde-4-O-beta-D-glucopyranoside
-
 ?
2.4.1.111 UDP-glucose + sinapyl alcohol glucosylated at 14% of the activity with coniferyl alcohol Picea abies UDP + syringin
-
 ?
2.4.1.111 UDP-glucose + vanillic acid glucosylated at 14% of the activity with coniferyl alcohol Picea abies UDP + vanillic acid-4-O-beta-D-glucopyranoside
-
 ?
2.4.1.111 UDP-glucose + vanillin
-
 Picea abies UDP + vanillin-4-O-beta-D-glucopyranoside
-
 ?
2.4.1.111 UDPglucose + coniferyl alcohol participates in lignification of spruce Picea abies ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.111 monomer 1 * 50000, SDS-PAGE Picea abies

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.111 36
-
-
 Picea abies

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.111 7.5
-
 Tris-HCl, 0.1-0.2 M, or phosphate buffer Picea abies

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.111 5.5 8.2 pH 5.5: about 50% of maximal activity, pH 8.2: about 70% of maximal activity, phosphate buffer Picea abies
2.4.1.111 6.5 8.2 about half-maximal activity at pH 6.5 and 83% of maximal activity at pH 8.2, Tris buffer Picea abies