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Literature summary extracted from

  • Kimura, M.; Okuno, E.; Inada, J.; Ohyama, H.; Kido, R.
    Purification and characterization of amino-acid N-choloyltransferase from human liver (1983), Hoppe-Seyler's Z. Physiol. Chem., 364, 637-645.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.65 EDTA 2fold activation, lowers the Km-value Homo sapiens
2.3.1.65 L-cysteine 2fold activation Homo sapiens
2.3.1.65 reduced glutathione 2fold activation Homo sapiens

General Stability

EC Number General Stability Organism
2.3.1.65 glycerol, 40% v/v, stabilizes during storage Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.65 chenodeoxycholate
-
 Homo sapiens
2.3.1.65 cholate
-
 Homo sapiens
2.3.1.65 cholesterol
-
 Homo sapiens
2.3.1.65 deoxycholate
-
 Homo sapiens
2.3.1.65 glycocholate
-
 Homo sapiens
2.3.1.65 glycodeoxycholate
-
 Homo sapiens
2.3.1.65 additional information not inhibited by 2-mercaptoethanol, CaCl2, EDTA, glutathione Homo sapiens
2.3.1.65 taurocholate
-
 Homo sapiens
2.3.1.65 taurodeoxycholate
-
 Homo sapiens
2.3.1.65 ursodeoxycholate
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.65 0.05
-
 choloyl-CoA + 60 mM glycine Homo sapiens
2.3.1.65 0.087
-
 choloyl-CoA + 40 mM taurine Homo sapiens
2.3.1.65 0.6
-
 taurine
-
 Homo sapiens
2.3.1.65 3.2
-
 glycine
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.65 52000
-
 2 * 52000, SDS-PAGE Homo sapiens
2.3.1.65 100000
-
 gel filtration Homo sapiens
2.3.1.65 118000
-
 sucrose density gradient centrifugation Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.65 choloyl-CoA + glycine Homo sapiens taurine can replace glycine, involved in conjugation of bile acids CoA + glycocholate
-
 ?
2.3.1.65 choloyl-CoA + glycine Homo sapiens cholesterol degradation, glycine conjugates of bile acids occur in adult CoA + glycocholate
-
 ?
2.3.1.65 choloyl-CoA + taurine Homo sapiens taurine can replace glycine, involved in conjugation of bile acids CoA + taurocholate
-
 ?
2.3.1.65 choloyl-CoA + taurine Homo sapiens cholesterol degradation, bile acids are secreted as taurine conjugates in fetus and neonatus CoA + taurocholate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.65 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.65 about 100fold Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.65 liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.65 0.42
-
 taurine Homo sapiens
2.3.1.65 0.43
-
 glycine Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.3.1.65 -20°C, in the presence of 40% v/v glycerol Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.65 chenodeoxycholoyl-CoA + glycine 91.4% of activity with choloyl-CoA Homo sapiens CoA + glycochenodeoxycholate
-
 ?
2.3.1.65 chenodeoxycholoyl-CoA + taurine 101.4% of activity with choloyl-CoA Homo sapiens CoA + taurochenodeoxycholate
-
 ?
2.3.1.65 choloyl-CoA + beta-alanine about 10% of the rate with glycine Homo sapiens CoA + beta-alanylcholate
-
 ?
2.3.1.65 choloyl-CoA + D-alpha-alanine about 10% of the rate with glycine Homo sapiens CoA + D-alpha-alanylcholate
-
 ?
2.3.1.65 choloyl-CoA + glycine ratio of glycine-/taurine-dependent activity is 1.0-1.4 Homo sapiens CoA + glycocholate
-
 ?
2.3.1.65 choloyl-CoA + glycine taurine can replace glycine, involved in conjugation of bile acids Homo sapiens CoA + glycocholate
-
 ?
2.3.1.65 choloyl-CoA + glycine cholesterol degradation, glycine conjugates of bile acids occur in adult Homo sapiens CoA + glycocholate
-
 ?
2.3.1.65 choloyl-CoA + taurine ratio of glycine-/taurine-dependent activity is 1.0-1.4 Homo sapiens CoA + taurocholate
-
 ?
2.3.1.65 choloyl-CoA + taurine taurine can replace glycine, involved in conjugation of bile acids Homo sapiens CoA + taurocholate
-
 ?
2.3.1.65 choloyl-CoA + taurine cholesterol degradation, bile acids are secreted as taurine conjugates in fetus and neonatus Homo sapiens CoA + taurocholate
-
 ?
2.3.1.65 deoxycholoyl-CoA + glycine 92.4% of activity with choloyl-CoA Homo sapiens CoA + glycodeoxycholate
-
 ?
2.3.1.65 deoxycholoyl-CoA + taurine 81.7% of activity with choloyl-CoA Homo sapiens CoA + taurodeoxycholate
-
 ?
2.3.1.65 lithocholoyl-CoA + glycine 58.4% of activity with choloyl-CoA Homo sapiens CoA + glycolithocholate
-
 ?
2.3.1.65 lithocholoyl-CoA + taurine 43% of activity with choloyl-CoA Homo sapiens CoA + taurolithocholate
-
 ?
2.3.1.65 additional information not: L-alpha-alanine, DL-cysteic acid, 4-aminobutyric acid, cysteine, sulfanilic acid Homo sapiens ?
-
 ?
2.3.1.65 ursodeoxycholoyl-CoA + glycine 24.8% of activity with choloyl-CoA Homo sapiens CoA + glycoursodeoxycholate
-
 ?
2.3.1.65 ursodeoxycholoyl-CoA + taurine 35.3% of activity with choloyl-CoA Homo sapiens CoA + tauroursodeoxycholate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.65 homodimer 2 * 52000, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.65 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.65 additional information
-
 pI: 5.85 Homo sapiens
2.3.1.65 6.5
-
 taurine-dependent activity Homo sapiens
2.3.1.65 7.2
-
 glycine-dependent activity Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.65 5.6 8.2 taurine: about half-maximal activity at pH 5.2 and pH 8.2, glycine: 80% of maximal activity at pH 8.2 Homo sapiens