Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Czuba, B.; Vessey, D.A.
    Kinetic characterization of cholyl-CoA glycine-taurine N-acyltransferase from bovine liver (1980), J. Biol. Chem., 255, 5296-5299.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.65 high ionic strength stabilizes Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.65 cholate
-
 Bos taurus
2.3.1.65 Conjugated bile acids efficient competitive inhibitors of cholyl-CoA binding Bos taurus
2.3.1.65 glycocholate competitive to cholyl-CoA, uncompetitive to glycine Bos taurus
2.3.1.65 additional information not inhibited by N-ethylmaleimide, iodoacetate Bos taurus
2.3.1.65 NaCl above 50 mM Bos taurus
2.3.1.65 p-mercuribenzoate reversible by dithioerythritol, substrates protect, serine and alanine protect Bos taurus
2.3.1.65 taurocholate
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.65 additional information
-
 additional information
-
 Bos taurus
2.3.1.65 0.0072
-
 choloyl-CoA
-
 Bos taurus
2.3.1.65 6.7
-
 taurine
-
 Bos taurus
2.3.1.65 8.8
-
 glycine
-
 Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.65 choloyl-CoA + glycine Bos taurus taurine can replace glycine, involved in conjugation of bile acids CoA + glycocholate
-
 ?
2.3.1.65 choloyl-CoA + taurine Bos taurus taurine can replace glycine, involved in conjugation of bile acids CoA + taurocholate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.65 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.65
-
 Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.65 choloyl-CoA + glycine = CoA + glycocholate ping-pong mechanism Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.65 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.65 43 59 glycine Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.65 chenodeoxycholoyl-CoA + glycine rate of conjugation in decreasing order: deoxycholyl-CoA, cholyl-CoA, lithocholyl-CoA, chenodeoxycholyl-CoA Bos taurus CoA + glycochenodeoxycholate
-
 ?
2.3.1.65 choloyl-CoA + glycine rate of conjugation in decreasing order: deoxycholyl-CoA, cholyl-CoA, lithocholyl-CoA, chenodeoxycholyl-CoA Bos taurus CoA + glycocholate
-
 ir
2.3.1.65 choloyl-CoA + glycine taurine can replace glycine, involved in conjugation of bile acids Bos taurus CoA + glycocholate
-
 ?
2.3.1.65 choloyl-CoA + taurine
-
 Bos taurus CoA + taurocholate
-
 ?
2.3.1.65 choloyl-CoA + taurine taurine can replace glycine, involved in conjugation of bile acids Bos taurus CoA + taurocholate
-
 ?
2.3.1.65 deoxycholoyl-CoA + glycine rate of conjugation in decreasing order: deoxycholyl-CoA, cholyl-CoA, lithocholyl-CoA, chenodeoxycholyl-CoA Bos taurus CoA + glycodeoxycholate
-
 ?
2.3.1.65 lithocholoyl-CoA + glycine rate of conjugation in decreasing order: deoxycholyl-CoA, cholyl-CoA, lithocholyl-CoA, chenodeoxycholyl-CoA Bos taurus CoA + glycolithocholate
-
 ?
2.3.1.65 additional information not: L-serine, beta-alanine Bos taurus ?
-
 ?
2.3.1.65 additional information not as acyl-CoA donors: acetyl-CoA, phenylacetyl-CoA and benzoyl-CoA Bos taurus ?
-
 ?
2.3.1.65 additional information not: L-alpha-alanine, L-glutamine, L-ornithine Bos taurus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.65 30
-
 assay at Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.65 additional information
-
 additional information higher kcat for glycine than for taurine Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.65 0.038
-
 glycocholate
-
 Bos taurus