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Literature summary extracted from

  • Sato, T.; Hoshino, T.
    Kinetic studies on the function of all the conserved tryptophans involved inside and outside the QW motifs of squalene-hopene cyclase: stabilizing effect of the protein structure against thermal denaturation (1999), Biosci. Biotechnol. Biochem., 63, 1171-1180.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.17
-
 Alicyclobacillus acidocaldarius

Protein Variants

EC Number Protein Variants Comment Organism
5.4.99.17 W23V same activity and optimal temperature as wild type enzyme Alicyclobacillus acidocaldarius
5.4.99.17 W258L 60% of wild type activity, lower temperature optimum Alicyclobacillus acidocaldarius
5.4.99.17 W406V no enzyme activity Alicyclobacillus acidocaldarius
5.4.99.17 W417A no enzyme activity Alicyclobacillus acidocaldarius
5.4.99.17 W485V same activity and optimal temperature as wild type enzyme Alicyclobacillus acidocaldarius
5.4.99.17 W522V same activity and optimal temperature as wild type enzyme Alicyclobacillus acidocaldarius
5.4.99.17 W533A same activity and optimal temperature as wild type enzyme Alicyclobacillus acidocaldarius
5.4.99.17 W591L same activity and optimal temperature as wild type enzyme Alicyclobacillus acidocaldarius
5.4.99.17 W78S same activity and optimal temperature as wild type enzyme Alicyclobacillus acidocaldarius

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.99.17 additional information
-
 additional information Km for wild type and mutant enzymes Alicyclobacillus acidocaldarius
5.4.99.17 0.0167
-
 squalene
-
 Alicyclobacillus acidocaldarius

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.17 Alicyclobacillus acidocaldarius
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.17
-
 Alicyclobacillus acidocaldarius

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4.99.17 additional information
-
 specific activities for wild type and mutant enzymes Alicyclobacillus acidocaldarius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.17 squalene
-
 Alicyclobacillus acidocaldarius hop-22(29)-ene
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.4.99.17 additional information
-
 wild type and mutant enzymes Alicyclobacillus acidocaldarius
5.4.99.17 55
-
 W258L mutant Alicyclobacillus acidocaldarius
5.4.99.17 60
-
-
 Alicyclobacillus acidocaldarius