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Literature summary extracted from
- Site-directed mutagenesis of squalene-hopene cyclase: altered substrate specificity and product distribution (2000), Chem. Biol., 7, 643-649.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.99.17 | - |
Alicyclobacillus acidocaldarius |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.4.99.17 | D377C/V380E/V381A | no detectable cyclization of squalene | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | E45A | reduced enzyme activity | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | E45D | reduced enzyme activity | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | E45K | no enzyme activity | Alicyclobacillus acidocaldarius |
| 5.4.99.17 | E45Q | slightly increased enzyme activity | Alicyclobacillus acidocaldarius |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.17 | additional information | - |
additional information | Km for wild type and mutant enzymes | Alicyclobacillus acidocaldarius |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.17 | Alicyclobacillus acidocaldarius | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.17 | squalene | - |
Alicyclobacillus acidocaldarius | hop-22(29)-ene | - |
? |  |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.17 | additional information | - |
wild type and mutant enzymes | Alicyclobacillus acidocaldarius |
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