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Literature summary extracted from

  • Qiao, N.; Takahashi, Y.; Takamatsu, H.; Yoshimoto, T.
    Leukotriene A synthase activity of purified mouse skin arachidonate 8-lipoxygenase expressed in Escherichia coli (1999), Biochim. Biophys. Acta, 1438, 131-139.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.40 skin 8-lipoxygenase expressed in COS-7 cells by transient transfection of its cDNA, amplified by PCR, in pEF-BOS, also expressed in Escherichia coli system using pQE-32 Mus musculus

General Stability

EC Number General Stability Organism
1.13.11.40 leukotriene A synthase activity of the enzyme leveled off within 10 min, indicating suicide activation Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.40 AA861
-
 Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.40 2 arachidonic acid + 2 O2 Mus musculus
-
 8-hydroxy,9-oxo-eicosa-5Z,11Z,14Z-trienoic acid + 9-oxo-[8,12-cis]-prosta-5Z,10,14Z-trienoic acid + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.40 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.40 recombinant enzyme Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.40 skin
-
 Mus musculus
-

Storage Stability

EC Number Storage Stability Organism
1.13.11.40 -70°C, crude and purified enzyme preparations can be stored without appreciable loss of activity for at least 6 months Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.40 2 arachidonic acid + 2 O2
-
 Mus musculus 8-hydroxy,9-oxo-eicosa-5Z,11Z,14Z-trienoic acid + 9-oxo-[8,12-cis]-prosta-5Z,10,14Z-trienoic acid + H2O
-
 ?
1.13.11.40 5-hydroperoxy fatty acid + O2 leukotriene A synthase activity, reaction rate is approximately 7% of arachidonate 8-lipoxygenation Mus musculus leukotriene A4
-
 ?
1.13.11.40 5-hydroperoxyeicosatetraenoic acid + ?
-
 Mus musculus leukotriene A4 unstable product ?
1.13.11.40 alpha-linolenic acid + O2
-
 Mus musculus ?
-
 ?
1.13.11.40 arachidonic acid + O2
-
 Mus musculus (8R)-8-hydroperoxy-5,9,11,14-eicosatetraenoic acid
-
 ?
1.13.11.40 docosahexaenoic acid + O2
-
 Mus musculus ?
-
 ?
1.13.11.40 eicosapentaenoic acid + O2
-
 Mus musculus ?
-
 ?
1.13.11.40 gamma-linoleic acid + O2
-
 Mus musculus ?
-
 ?
1.13.11.40 linoleic acid + O2 poor substrate Mus musculus ?
-
 ?