Literature summary extracted from
M'Baya, B.; Karst, F.
In vitro assay of squalene epoxidase of Saccharomyces cerevisiae (1987), Biochem. Biophys. Res. Commun., 147, 556-564.
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
1.14.14.17 |
H2O2 |
inhibition above 2 mM |
Saccharomyces cerevisiae |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
1.14.14.17 |
0.0043 |
- |
O2 |
- |
Saccharomyces cerevisiae |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.14.14.17 |
Saccharomyces cerevisiae |
- |
enzyme is depressed in anaerobically grown cells |
- |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
1.14.14.17 |
0.0001 |
- |
activity in cell-free extract |
Saccharomyces cerevisiae |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
1.14.14.17 |
-25°C, overnight, 2fold decrease of activity in crude extracts |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.14.14.17 |
squalene + NAD(P)H + O2 |
NADPH is preferred |
Saccharomyces cerevisiae |
(S)-squalene-2,3-epoxide + NADP+ + H2O |
- |
? |
|
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
1.14.14.17 |
30 |
37 |
activity in crude extract |
Saccharomyces cerevisiae |