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Literature summary extracted from

  • Kastrau, D.H.W.; Heiss, B.; Kroneck, P.M.H.; Zumft, W.G.
    Nitric oxide reductase from Pseudomonas stutzeri, a novel cytochrome bc complex. Phospholipid requirement, electron paramagnetic resonance and redox properties (1994), Eur. J. Biochem., 222, 293-303.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.7.2.5 cardiolipin
-
 Pseudomonas stutzeri
1.7.2.5 phosphatidylglycerol
-
 Pseudomonas stutzeri

General Stability

EC Number General Stability Organism
1.7.2.5 a phospholipid is required for high catalytic activity Pseudomonas stutzeri

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.2.5 elaidic acid
-
 Pseudomonas stutzeri
1.7.2.5 linoleic acid
-
 Pseudomonas stutzeri
1.7.2.5 linolenic acid
-
 Pseudomonas stutzeri
1.7.2.5 oleic acid
-
 Pseudomonas stutzeri
1.7.2.5 palmitoleic acid
-
 Pseudomonas stutzeri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.2.5 Fe
-
 Pseudomonas stutzeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.2.5 17000
-
 1 * 17000 + 1 * 38000, SDS-PAGE Pseudomonas stutzeri
1.7.2.5 38000
-
 1 * 17000 + 1 * 38000, SDS-PAGE Pseudomonas stutzeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.2.5 2 nitric oxide + reduced acceptor Pseudomonas stutzeri
-
 nitrous oxide + acceptor + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.5 Pseudomonas stutzeri
-
 ZoBell strain, ATCC 14405
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.7.2.5 lipoprotein contains cardiolipin, phosphatidylglycerol and phosphatidylethanolamine Pseudomonas stutzeri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.2.5 60
-
 ascorbate and phenazine methosulfate as electron donors Pseudomonas stutzeri

Storage Stability

EC Number Storage Stability Organism
1.7.2.5 17°C, 0.1% laurylpropanediol-3-phosphorylcholine ether or 0.1% dodecylmaltoside, 8 days, 100% activity Pseudomonas stutzeri
1.7.2.5 17°C, Triton X-100, 2 days, 100% activity Pseudomonas stutzeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.5 2 nitric oxide + reduced acceptor
-
 Pseudomonas stutzeri nitrous oxide + acceptor + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.2.5 dimer 1 * 17000 + 1 * 38000, SDS-PAGE Pseudomonas stutzeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.2.5 4.8
-
-
 Pseudomonas stutzeri

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.7.2.5 4.8 6 at pH 6.0 half of the activity of pH 4.8 Pseudomonas stutzeri

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.7.2.5 6.5
-
 inactivation after 1 h at room temperature Pseudomonas stutzeri

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.2.5 heme
-
 Pseudomonas stutzeri

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.7.2.5 0.0065
-
 oleic acid
-
 Pseudomonas stutzeri
1.7.2.5 0.0098
-
 palmitoleic acid
-
 Pseudomonas stutzeri
1.7.2.5 0.0125
-
 linoleic acid
-
 Pseudomonas stutzeri
1.7.2.5 0.014
-
 linolenic acid
-
 Pseudomonas stutzeri
1.7.2.5 0.0235
-
 elaidic acid
-
 Pseudomonas stutzeri