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Literature summary extracted from

  • Matsuda, H.; Suzuki, Y.
    Purification and properties of the diamine oxidase from Vicia faba leaves (1981), Plant Cell Physiol., 22, 737-746.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.22 1,4-phenanthroline 0.1 mM Vicia faba
1.4.3.22 Cuprizone 0.1 mM Vicia faba
1.4.3.22 diethyldithiocarbamate
-
 Vicia faba
1.4.3.22 phenylhydrazine
-
 Vicia faba

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.3.22 copper 2 copper atoms per mol of enzyme Vicia faba

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.22 74000
-
 2 * 74000, SDS-PAGE Vicia faba
1.4.3.22 126000
-
 gel filtration Vicia faba

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.22 Vicia faba
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.22
-
 Vicia faba

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.22 leaf
-
 Vicia faba
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.22 21.6
-
-
 Vicia faba

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.22 1,4-diaminobutane + H2O + O2
-
 Vicia faba 4-aminobutanal + NH3 + H2O2
-
 ?
1.4.3.22 1,5-diaminopentane + H2O + O2
-
 Vicia faba 5-aminopentanal + NH3 + H2O2
-
 ?
1.4.3.22 2-phenylethylamine + H2O + O2
-
 Vicia faba 2-phenylethanal + NH3 + H2O2
-
 ?
1.4.3.22 agmatine
-
 Vicia faba ?
-
 ?
1.4.3.22 benzylamine + H2O + O2
-
 Vicia faba benzaldehyde + NH3 + H2O2
-
 ?
1.4.3.22 histamine + H2O + O2
-
 Vicia faba (imidazol-4-yl)acetaldehyde + NH3 + H2O2
-
 ?
1.4.3.22 lysine + H2O + O2
-
 Vicia faba 2-aminohexanoic acid + NH3 + H2O2
-
 ?
1.4.3.22 RCH2NH2 + H2O + O2
-
 Vicia faba RCHO + NH3 + H2O2
-
 ?
1.4.3.22 spermidine + H2O + O2
-
 Vicia faba ?
-
 ?
1.4.3.22 spermine + H2O + O2
-
 Vicia faba ?
-
 ?
1.4.3.22 tryptamine
-
 Vicia faba ?
-
 ?
1.4.3.22 tyramine + H2O + O2
-
 Vicia faba 4-hydroxyphenylethanal + NH3 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.22 dimer 2 * 74000, SDS-PAGE Vicia faba

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.22 40
-
-
 Vicia faba

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.4.3.22 20 63 about 50% activity at 20°C and 63°C Vicia faba

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.22
-
 60 10 min, pH 7.0, stable Vicia faba
1.4.3.22 70
-
 10 min, pH 7, 100% loss of activity Vicia faba

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.22 7
-
 cadaverine, histamine, agmatine Vicia faba
1.4.3.22 7.2
-
 putrescine, spermidine, tryptamine Vicia faba
1.4.3.22 7.7
-
 tyramine Vicia faba
1.4.3.22 8.5
-
 dopamine, phenylethylamine, benzylamine Vicia faba
1.4.3.22 8.6
-
 lysine Vicia faba

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.3.22 3
-
 4 h, complete loss of activity Vicia faba
1.4.3.22 3.5
-
 4 h, 50% loss of activity Vicia faba
1.4.3.22 5 9 30°C, stable Vicia faba
1.4.3.22 11
-
 4 h, 50% loss of activity Vicia faba

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.22 additional information 1 mol carbonyl-group per mol enzyme Vicia faba