EC Number | General Stability | Organism |
---|---|---|
1.4.1.14 | 2-mercaptoethanol stabilizes | Saccharomyces cerevisiae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.13 | L-2-Amino-4-oxo-5-chloropentanoic acid | selective inhibitor of glutamine-dependent activity | Escherichia coli | |
1.4.1.13 | L-2-Amino-4-oxo-5-chloropentanoic acid | selective inhibitor of glutamine-dependent activity | Klebsiella aerogenes | |
1.4.1.14 | (2S)-2-amino-4-(S-methylsulfonimidoyl)butanoic acid | - |
Saccharomyces cerevisiae | |
1.4.1.14 | D-glutamine | - |
Saccharomyces cerevisiae | |
1.4.1.14 | DL-ethionine sulfone | - |
Saccharomyces cerevisiae | |
1.4.1.14 | DL-homocysteic acid | - |
Saccharomyces cerevisiae | |
1.4.1.14 | L-Albizziin | - |
Saccharomyces cerevisiae | |
1.4.1.14 | L-homocysteine sulfonamide | very potent transition state inhibitor, competitive to L-glutamine | Saccharomyces cerevisiae | |
1.4.1.14 | L-methionine sulfone | markedly inhibits | Saccharomyces cerevisiae | |
1.4.1.14 | L-methionine-SR-sulfoxide | - |
Saccharomyces cerevisiae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.13 | 0.012 | - |
NADPH | - |
Klebsiella aerogenes | |
1.4.1.13 | 0.3 | - |
L-glutamine | - |
Klebsiella aerogenes | |
1.4.1.13 | 0.3 | - |
alpha-ketoglutarate | - |
Klebsiella aerogenes | |
1.4.1.13 | 0.5 | 2 | NH4Cl | - |
Klebsiella aerogenes | |
1.4.1.14 | 0.007 | - |
NADH | - |
Saccharomyces cerevisiae | |
1.4.1.14 | 0.04 | - |
2-oxoglutarate | - |
Saccharomyces cerevisiae | |
1.4.1.14 | 0.28 | - |
L-glutamine | - |
Saccharomyces cerevisiae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.4.1.13 | 51500 | - |
x * 175000 + x * 51500, SDS-PAGE | Klebsiella aerogenes |
1.4.1.13 | 175000 | - |
x * 175000 + x * 51500, SDS-PAGE | Klebsiella aerogenes |
1.4.1.14 | 61000 | - |
1 * 169000 + 1 * 61000, SDS-PAGE in presence of 1% 2-mercaptoethanol | Saccharomyces cerevisiae |
1.4.1.14 | 169000 | - |
1 * 169000 + 1 * 61000, SDS-PAGE in presence of 1% 2-mercaptoethanol | Saccharomyces cerevisiae |
1.4.1.14 | 265000 | - |
gel filtration | Saccharomyces cerevisiae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.1.13 | Escherichia coli | - |
- |
- |
1.4.1.13 | Klebsiella aerogenes | - |
- |
- |
1.4.1.14 | Saccharomyces cerevisiae | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.1.13 | using sonication, heat treatment, protamine sulfate precipitation, ammonium sulfate precipitation, gel filtration on G-50, column chromatography on DEAE-Sephadex and gel filtration on Sephadex G-200 and Sepharose 6B | Klebsiella aerogenes |
1.4.1.14 | - |
Saccharomyces cerevisiae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.4.1.13 | additional information | - |
- |
Escherichia coli |
1.4.1.13 | additional information | - |
- |
Klebsiella aerogenes |
1.4.1.13 | 13.33 | - |
- |
Escherichia coli |
1.4.1.13 | 20.83 | - |
- |
Klebsiella aerogenes |
1.4.1.14 | 41.7 | - |
- |
Saccharomyces cerevisiae |
EC Number | Storage Stability | Organism |
---|---|---|
1.4.1.13 | 4°C, 200 mM potassium phosphate buffer, pH 7.6, 0.5 mM Na2EDTA | Klebsiella aerogenes |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.13 | L-glutamine + 2-oxoglutarate + NADPH + H+ | in the reverse reaction ammonia can act instead of glutamine, but more slowly | Escherichia coli | L-glutamate + NADP+ | - |
? | |
1.4.1.13 | L-glutamine + 2-oxoglutarate + NADPH + H+ | in the reverse reaction ammonia can act instead of glutamine, but more slowly | Klebsiella aerogenes | L-glutamate + NADP+ | - |
? | |
1.4.1.13 | additional information | - |
Escherichia coli | ? | - |
? | |
1.4.1.13 | additional information | glutamine binding site of the enzyme is located on the heavy subunit of the enzyme, preparations of the enzyme that lack flavins or the flavins and iron sulfide catalyze NH3-dependent reaction but not glutamine-dependent reaction | Klebsiella aerogenes | ? | - |
? | |
1.4.1.13 | NH3 + 2-oxoglutarate + NADPH + H+ | - |
Escherichia coli | L-glutamate + NADP+ | - |
? | |
1.4.1.13 | NH3 + 2-oxoglutarate + NADPH + H+ | - |
Klebsiella aerogenes | L-glutamate + NADP+ | - |
? | |
1.4.1.14 | L-glutamine + 2-oxoglutarate + NADH | low activity when NH3 is substituted for glutamine, NH4Cl: 2.7% of the activity with L-glutamine | Saccharomyces cerevisiae | L-glutamate + NAD+ | - |
? | |
1.4.1.14 | L-glutamine + 2-oxoglutarate + NADH | specific for NADH | Saccharomyces cerevisiae | L-glutamate + NAD+ | - |
? | |
1.4.1.14 | additional information | no activity with 2-oxovalerate, 2-oxopimelate, 2-oxoadipate, oxaloacetate, D-glutamine, L- or D-asparagine, L- or D-homoglutamine, alpha-methyl-L-glutamine, DL-beta-glutamine, L-isoglutamine, glutaramide or glutaramate | Saccharomyces cerevisiae | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.4.1.13 | ? | x * 175000 + x * 51500, SDS-PAGE | Klebsiella aerogenes |
1.4.1.14 | dimer | 1 * 169000 + 1 * 61000, SDS-PAGE in presence of 1% 2-mercaptoethanol | Saccharomyces cerevisiae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.4.1.13 | 7.8 | - |
glutamine-dependent activity | Klebsiella aerogenes |
1.4.1.13 | 8 | - |
glutamine-dependent activity | Klebsiella aerogenes |
1.4.1.13 | 9 | - |
ammonia-dependent activity | Klebsiella aerogenes |
1.4.1.14 | 7 | 7.5 | glutamine-dependent NADH oxidase activity | Saccharomyces cerevisiae |
1.4.1.14 | 9 | - |
ammonia-dependent NADH oxidase activity, rate steadily increases from pH 6 to 9 | Saccharomyces cerevisiae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.13 | NADH | no activity with NADH | Escherichia coli | |
1.4.1.13 | NADH | no activity with NADH | Klebsiella aerogenes | |
1.4.1.13 | NADPH | - |
Escherichia coli | |
1.4.1.13 | NADPH | - |
Klebsiella aerogenes | |
1.4.1.14 | additional information | no activity with NADPH | Saccharomyces cerevisiae | |
1.4.1.14 | NADH | specific for NADH as reductant | Saccharomyces cerevisiae |