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Literature summary extracted from

  • Schreier, H.J.; Bernlohr, R.W.
    Purification and properties of glutamate synthase from Bacillus licheniformis (1984), J. Bacteriol., 160, 591-599.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.13 CaCl2 27% inhibition at 50 mM Bacillus licheniformis
1.4.1.13 DL-methionine sulfoxide potent inhibitor Bacillus licheniformis
1.4.1.13 glycine 5% inhibition at 5 mM Bacillus licheniformis
1.4.1.13 L-alanine 5% inhibition at 5 mM Bacillus licheniformis
1.4.1.13 L-histidine 5% inhibition at 5 mM Bacillus licheniformis
1.4.1.13 L-leucine 16% inhibition at 5 mM Bacillus licheniformis
1.4.1.13 L-methionine 16% inhibition at 5 mM Bacillus licheniformis
1.4.1.13 L-methionine DL-sulfoximine potent inhibitor Bacillus licheniformis
1.4.1.13 L-methionine sulfone potent inhibitor Bacillus licheniformis
1.4.1.13 L-serine 10% inhibition at 5 mM Bacillus licheniformis
1.4.1.13 MgCl2 31% inhibition at 50 mM Bacillus licheniformis
1.4.1.13 MnCl2 64% inhibition at 50 mM Bacillus licheniformis
1.4.1.13 additional information relatively insensitive to inhibition by amino acids, keto acids or various nucleotides, 38% inhibition with a combination of each 5 mM L-methionine, L-leucine, L-serine, L-histidine, L-glycine and L-alanine Bacillus licheniformis
1.4.1.13 NADP+ competitive inhibition with respect to NADPH; potent inhibitor Bacillus licheniformis
1.4.1.13 NADPH
-
Bacillus licheniformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.13 0.013
-
NADPH
-
Bacillus licheniformis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.1.13 Iron iron-sulfur protein Bacillus licheniformis
1.4.1.13 Iron 8.1 iron atoms per MW 220000 Bacillus licheniformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.13 54000
-
1 * 158000 + 1 * 54000, SDS-PAGE Bacillus licheniformis
1.4.1.13 158000
-
1 * 158000 + 1 * 54000, SDS-PAGE Bacillus licheniformis
1.4.1.13 220000
-
sucrose density gradient sedimentation Bacillus licheniformis

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.13 Bacillus licheniformis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.13 using streptomycin sulfate treatment, ammonium sulfate precipitation, column chromatography on DEAE-Sephacel, Bio-Gel A 1.5 m, Red Sepharose CL6B and Sephadex G-25 Bacillus licheniformis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.13 22.9
-
-
Bacillus licheniformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.13 L-glutamine + 2-oxoglutarate + NADPH + H+ highly specific Bacillus licheniformis L-glutamate + NADP+
-
?
1.4.1.13 L-glutamine + 2-oxoglutarate + NADPH + H+ in the reverse reaction ammonia can act instead of glutamine, but more slowly Bacillus licheniformis L-glutamate + NADP+
-
?

Subunits

EC Number Subunits Comment Organism
1.4.1.13 dimer 1 * 158000 + 1 * 54000, SDS-PAGE Bacillus licheniformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.13 7.8
-
glutamine-dependent activity Bacillus licheniformis
1.4.1.13 8.7
-
ammonia-dependent activity Bacillus licheniformis

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.13 flavin ratio FAD: FMN is 1.2 Bacillus licheniformis
1.4.1.13 flavin 2 flavin moieties per 220000 MW Bacillus licheniformis
1.4.1.13 additional information the enzyme contains 8.1 acid-labile sulfur atoms per 220000-dalton dimer Bacillus licheniformis
1.4.1.13 NADPH
-
Bacillus licheniformis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.1.13 0.02
-
NADP competitive inhibition with NADPH Bacillus licheniformis