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Literature summary extracted from

  • Owaki, J.; Uzura, K.; Minami, Z.; Kuai, K.
    Partial purification and characterization of dihydrouracil oxidase, a flavoprotein from Rhodotorula glutinis (1986), J. Ferment. Technol., 64, 205-210.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.3.7 5-Bromouracil competitive vs. dihydrouracil Rhodotorula glutinis
1.3.3.7 5-Chlorouracil competitive vs. dihydrouracil Rhodotorula glutinis
1.3.3.7 5-fluorouracil competitive vs. dihydrouracil Rhodotorula glutinis
1.3.3.7 5-Iodouracil competitive vs. dihydrouracil Rhodotorula glutinis
1.3.3.7 5-Nitrouracil competitive vs. dihydrouracil Rhodotorula glutinis
1.3.3.7 p-chloromercuribenzoate
-
 Rhodotorula glutinis
1.3.3.7 thymine competitive vs. dihydrouracil Rhodotorula glutinis
1.3.3.7 Uracil competitive vs. dihydrouracil Rhodotorula glutinis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.3.7 0.05
-
 dihydrouracil
-
 Rhodotorula glutinis
1.3.3.7 0.05
-
 Dihydrothymine
-
 Rhodotorula glutinis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.3.7 80000
-
 gel filtration Rhodotorula glutinis

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.7 Rhodotorula glutinis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.3.7 ammonium sulfate, DEAE-cellulose, DEAE-Sephadex a-50, electrofocusing, Sephadex G-200, partially purified Rhodotorula glutinis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.3.7 9.25
-
-
 Rhodotorula glutinis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.7 5,6-dihydrouracil + O2 O2 is the only known electron acceptor, no substrates are dihydro-6-methyluracil, dihydro D-orotic acid and dihydro L-orotic acid Rhodotorula glutinis uracil + H2O2
-
 ?
1.3.3.7 dihydrothymine + O2
-
 Rhodotorula glutinis thymine + H2O2
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.3.7 7 8
-
 Rhodotorula glutinis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.3.3.7 30
-
 stable up to Rhodotorula glutinis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.3.7 7 8
-
 Rhodotorula glutinis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.3.3.7 6 10 1 h, 25°C, stable Rhodotorula glutinis

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.3.7 FMN flavoprotein Rhodotorula glutinis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.3.7 0.003
-
 thymine
-
 Rhodotorula glutinis
1.3.3.7 0.0035
-
 5-Nitrouracil
-
 Rhodotorula glutinis
1.3.3.7 0.01
-
 5-fluorouracil
-
 Rhodotorula glutinis
1.3.3.7 0.02
-
 5-Chlorouracil
-
 Rhodotorula glutinis
1.3.3.7 0.032
-
 5-Bromouracil
-
 Rhodotorula glutinis
1.3.3.7 0.035
-
 Uracil
-
 Rhodotorula glutinis
1.3.3.7 0.088
-
 5-Iodouracil
-
 Rhodotorula glutinis