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Literature summary extracted from

  • Penzes, P.; Wang, X.; Napoli, J.L.
    Enzymic characteristics of retinal dehydrogenase type I expressed in Escherichia coli (1997), Biochim. Biophys. Acta, 1342, 175-181.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.36 CRBP enzyme recognizes retinal as substrate in presence of CRBP Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.36
-
 Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.36 apo-CRBP IC50 0.0014 mM Rattus norvegicus
1.2.1.36 citral IC50 about 0.001 mM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.36 0.0003
-
 benzaldehyde
-
 Rattus norvegicus
1.2.1.36 0.0003
-
 hexanal
-
 Rattus norvegicus
1.2.1.36 0.0003
-
 decanal
-
 Rattus norvegicus
1.2.1.36 0.0003
-
 octanal
-
 Rattus norvegicus
1.2.1.36 0.0017
-
 all-trans-retinal
-
 Rattus norvegicus
1.2.1.36 0.0066
-
 propanal
-
 Rattus norvegicus
1.2.1.36 0.022
-
 NAD+ standard deviation 0.004 mM Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.36 cytosol
-
 Rattus norvegicus 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.36 retinal + NAD+ + H2O Rattus norvegicus
-
 retinoate + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.36 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.36
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.36 liver
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
1.2.1.36 -70°C, 20 mM Hepes, 2 mM DTT, 150 mM KCl, 15% glycerol, pH 8 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.36 benzaldehyde + NAD+ + H2O
-
 Rattus norvegicus benzoate + NADH + H+
-
 ?
1.2.1.36 decanal + NAD+ + H2O
-
 Rattus norvegicus decanoic acid + NADH
-
 ?
1.2.1.36 hexanal + NAD+ + H2O
-
 Rattus norvegicus hexanoate + NADH + H+
-
 ?
1.2.1.36 octanal + NAD+ + H2O
-
 Rattus norvegicus octanoate + NADH
-
 ?
1.2.1.36 propanal + NAD+ + H2O
-
 Rattus norvegicus propanoate + NADH + H+
-
 ?
1.2.1.36 retinal + NAD+ + H2O
-
 Rattus norvegicus retinoate + NADH
-
 ?

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.36 7.5 9 exhibits a broad pH optimum Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.36 NAD+ reaction is NAD+ dependent, rather than NADP+ dependent Rattus norvegicus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.2.1.36 0.001
-
 IC50 about 0.001 mM Rattus norvegicus citral
1.2.1.36 0.0014
-
 IC50 0.0014 mM Rattus norvegicus apo-CRBP