Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Livingstone, A.; Fewson, C.A.; Kennedy, S.I.T.; Zatman, L.J.
    Two benzaldehyde dehydrogenases in bacterium N.C.I.B. 8250. Distinguishing properties and regulation (1972), Biochem. J., 130, 927-935.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.28 4-chloromercuribenzoate 0.5 mM, complete inhibition Acinetobacter calcoaceticus
1.2.1.28 N-ethylmaleimide 0.5 mM, complete inhibition Acinetobacter calcoaceticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.28 Cs+ benzaldehyde dehydrogenase I Acinetobacter calcoaceticus
1.2.1.28 K+ or NH4+ or Rb+ required for maximal activity, maximum activity occurs at approx. 70 mM K+ Acinetobacter calcoaceticus
1.2.1.28 K+ only dehydrogenase I of strain N.C.I.B. 8250 is activated by univalent cations Acinetobacter calcoaceticus
1.2.1.28 Li+ benzaldehyde dehydrogenase I Acinetobacter calcoaceticus
1.2.1.28 Na+ benzaldehyde dehydrogenase I Acinetobacter calcoaceticus
1.2.1.28 NH4+ benzaldehyde dehydrogenase I Acinetobacter calcoaceticus
1.2.1.28 Rb+ benzaldehyde dehydrogenase I Acinetobacter calcoaceticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.28 180000
-
 strain N.C.I.B. 8250, gel filtration Acinetobacter calcoaceticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.28 benzaldehyde + NAD+ + H2O Acinetobacter calcoaceticus
-
 benzoate + NADH + H+
-
 ?
1.2.1.28 benzaldehyde + NAD+ + H2O Acinetobacter calcoaceticus N.C.I.B. 8250
-
 benzoate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.28 Acinetobacter calcoaceticus
-
 N.C.I.B. 8250
-
1.2.1.28 Acinetobacter calcoaceticus N.C.I.B. 8250
-
 N.C.I.B. 8250
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.28 dehydrogenases I and II Acinetobacter calcoaceticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.28 0.002
-
 benzaldehyde dehydrogenase II and class 1 mutant activity in cell extracts of wild-type grown without inducer Acinetobacter calcoaceticus
1.2.1.28 0.006
-
 class 1 mutant enzyme activity in cell extracts of wild-type grown in the presence of 5 mM benzyl alcohol or 1 mM benzaldehyde Acinetobacter calcoaceticus
1.2.1.28 0.094
-
 class 2 mutant enzyme activity in cell extracts of wild-type grown in the presence of 1 mM benzaldehyde Acinetobacter calcoaceticus
1.2.1.28 0.126
-
 benzaldehyde dehydrogenase II activity in cell extracts of wild-type grown in the presence of 1 mM benzaldehyde Acinetobacter calcoaceticus
1.2.1.28 0.324
-
 benzaldehyde dehydrogenase II activity in cell extracts of wild-type grown in the presence of 5 mM benzyl alcohol Acinetobacter calcoaceticus
1.2.1.28 0.325
-
 class 2 mutant enzyme activity in cell extracts of wild-type grown in the presence of 5 mM benzyl alcohol Acinetobacter calcoaceticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.28 benzaldehyde + NAD+ + H2O
-
 Acinetobacter calcoaceticus benzoate + NADH + H+
-
 ?
1.2.1.28 benzaldehyde + NAD+ + H2O
-
 Acinetobacter calcoaceticus N.C.I.B. 8250 benzoate + NADH + H+
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.28 37
-
 half-lives: benzaldehyde dehydrogenase I, 520 min, benzaldehyde dehydrogenase II, 30 min, pH 9.18, half-life for benzaldehyde dehydrogenase II increases to 113 min at pH 8.5 Acinetobacter calcoaceticus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.28 NAD+
-
 Acinetobacter calcoaceticus