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Literature summary extracted from

  • Garcia-Dominguez, M.; Reyes, J.C.; Florencio, F.J.
    Purification and characterization of a new type of glutamine synthetase from cyanobacteria (1997), Eur. J. Biochem., 244, 258-264.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.1.2 glnN gene overexpressed in Escherichia coli Synechocystis sp.

General Stability

EC Number General Stability Organism
6.3.1.2 strongly stabilized in presence of Mn2+ but not with other divalent cations. Maximal stabilization at 1 mM Synechocystis sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.1.2 ADP strong, enzyme form GSIII Synechocystis sp.
6.3.1.2 L-methionine sulfoximine enzyme form GS III Synechocystis sp.
6.3.1.2 Phosphinothricin
-
Synechocystis sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.1.2 0.19
-
NH4+ enzyme form GSIII Synechocystis sp.
6.3.1.2 0.43
-
ATP enzyme form GSIII Synechocystis sp.
6.3.1.2 0.9
-
Glu enzyme form GSIII Synechocystis sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.1.2 52000
-
12 * 52000, enzyme form GSI, SDS-PAGE Synechocystis sp.
6.3.1.2 80000
-
6 * 80000, enzyme form GSIII, SDS-PAGE Synechocystis sp.
6.3.1.2 500000
-
enzyme form GSIII, gel filtration Synechocystis sp.
6.3.1.2 624000
-
enzyme form GSI, gel filtration Synechocystis sp.

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.2 Synechocystis sp.
-
strain PCC 6803
-
6.3.1.2 Synechocystis sp.
-
enzyme form GSI and GSIII encoded by the gene glnA and glnB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.2 enzyme form GSIII Synechocystis sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.1.2 36.8
-
-
Synechocystis sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.2 ATP + L-Glu + NH4+
-
Synechocystis sp. ADP + phosphate + L-Gln
-
?

Subunits

EC Number Subunits Comment Organism
6.3.1.2 dodecamer 12 * 52000, enzyme form GSI, SDS-PAGE Synechocystis sp.
6.3.1.2 hexamer 6 * 80000, enzyme form GSIII, SDS-PAGE Synechocystis sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.1.2 35
-
enzyme form GSI, biosynthetic assay Synechocystis sp.
6.3.1.2 42
-
enzyme form GSIII, biosynthetic assay Synechocystis sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.1.2 50
-
20 min, complete inactivation Synechocystis sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.1.2 7.2
-
enzyme form GSI Synechocystis sp.
6.3.1.2 8.3
-
enzyme form GSIII, biosynthetic assay Synechocystis sp.