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Literature summary extracted from

  • Wajant, H.; Foerster, S.; Selmar, D.; Effenberger, F.; Pfizenmaier, K.
    Purification and characterization of a novel (R)-mandelonitrile lyase from the fern Phlebodium aureum (1995), Plant Physiol., 109, 1231-1238.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.10 AgNO3 0.2 mM, 85% inhibition Prunus dulcis
4.1.2.10 diethyl dicarbonate 0.5 mM, 60% inhibition Phlebodium aureum
4.1.2.10 diethyl dicarbonate 0.5 mM, 50% inhibition. 2 mM, 79% inhibition Prunus dulcis
4.1.2.10 diisopropyl fluorophosphate 2 mM, 80% inhibition Prunus dulcis
4.1.2.10 iodoacetamide 2 mM, 70% inhibition Prunus dulcis
4.1.2.10 additional information the enzyme is not inhibited by sulfhydryl- or hydroxyl-modifying reagents Phlebodium aureum
4.1.2.10 phenylmethylsulfonyl fluoride 2 mM, 50% inhibition Prunus dulcis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.10 0.83
-
 benzaldehyde pH 5.3 Phlebodium aureum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.10 20000
-
 x * 20000, SDS-PAGE Phlebodium aureum
4.1.2.10 168000
-
 isoenzyme A and C, gel filtration Phlebodium aureum

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.10 Phlebodium aureum
-
-
-
4.1.2.10 Prunus dulcis O24243
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.10
-
 Phlebodium aureum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.10 cyanide + benzaldehyde
-
 Phlebodium aureum (R)-mandelonitrile 82% yield, 99% enantiomeric excess ?
4.1.2.10 cyanide + benzaldehyde
-
 Prunus dulcis (R)-mandelonitrile 99% yield, 99% enantiomeric excess ?
4.1.2.10 cyanide + butanal
-
 Prunus dulcis (2R)-2-hydroxypentanenitrile 100% yield, 90% enantiomeric excess ?
4.1.2.10 cyanide + butanal
-
 Phlebodium aureum (2R)-2-hydroxypentanenitrile 42% yield, 55% enantiomeric excess ?
4.1.2.10 cyanide + pentan-2-one
-
 Prunus dulcis (2R)-2-hydroxy-2-methylpentanenitrile
-
 ?
4.1.2.10 cyanide + pentan-2-one
-
 Phlebodium aureum (2R)-2-hydroxy-2-methylpentanenitrile 17% yield ?
4.1.2.10 cyanide + thiophene-2-carbaldehyde
-
 Prunus dulcis (2R)-hydroxy(thiophen-2-yl)ethanenitrile 48% yield, 87% enantiomeric excess ?
4.1.2.10 cyanide + thiophene-2-carbaldehyde
-
 Phlebodium aureum (2R)-hydroxy(thiophen-2-yl)ethanenitrile 87% yield, 99% enentiomeric excess ?
4.1.2.10 additional information aliphatic carbonyls are poorly converted Phlebodium aureum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.10 multimer x * 20000, SDS-PAGE Phlebodium aureum

Synonyms

EC Number Synonyms Comment Organism
4.1.2.10 (R)-Mandelonitrile lyase
-
 Phlebodium aureum
4.1.2.10 (R)-Mandelonitrile lyase
-
 Prunus dulcis
4.1.2.10 PaMDL
-
 Prunus dulcis
4.1.2.10 PhaMDL
-
 Phlebodium aureum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.10 35 40
-
 Phlebodium aureum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.10 3.5 7 because of the base-catalyzed decomposition of mandelonitrile, pH dependence is only determined below pH 7.5. Activity increases from pH 3.5 to 6.2 and levels off between pH 6.2 and 7 for both isoforms Phlebodium aureum

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.2.10 FAD a flavoprotein Prunus dulcis
4.1.2.10 additional information not a flavoprotein Phlebodium aureum