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Literature summary extracted from

  • Neumann, A.; Siebert, A.; Trescher, T.; Reinhardt, S.; Wohlfarth, G.; Diekert, G.
    Tetrachloroethene reductive dehalogenase of Dehalospirillum multivorans: substrate specificity of the native enzyme and its corrinoid cofactor (2002), Arch. Microbiol., 177, 420-426.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.21.99.5 analysis reductive dehalogenation assay can be used for the sensitive and rapid quantification of the corrinoid cofactor Sulfurospirillum multivorans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.21.99.5 cis-1,3-dichloropropene 50% inhibition Sulfurospirillum multivorans
1.21.99.5 additional information no inhibition by 1-chloropropene, 2-chloropropene, 3-chloropropene, 1,1-dichloropropene, 1,2-dichloropropane, 1,3-dichloropropane, and 1,2,3-trichloropropane Sulfurospirillum multivorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.21.99.5 additional information
-
 additional information enzyme kinetics and kinetic parameters Sulfurospirillum multivorans
1.21.99.5 0.2
-
 tetrachloroethene reduced 1.6 mM methyl viologen as electron donor Sulfurospirillum multivorans
1.21.99.5 0.24
-
 trichloroethene reduced 1.6 mM methyl viologen as electron donor Sulfurospirillum multivorans
1.21.99.5 0.25
-
 trans-1,3-dichloropropene reduced 1.6 mM methyl viologen as electron donor Sulfurospirillum multivorans
1.21.99.5 0.25
-
 1,1,3-trichloropropene reduced 1.6 mM methyl viologen as electron donor Sulfurospirillum multivorans
1.21.99.5 0.28
-
 methyl viologen at a tetrachloroethene concentration of 1 mM Sulfurospirillum multivorans
1.21.99.5 0.6
-
 2,3-dichloropropene reduced 1.6 mM methyl viologen as electron donor Sulfurospirillum multivorans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.21.99.5 Fe2+ two Fe/S clusters Sulfurospirillum multivorans
1.21.99.5 Iron-sulfur cluster two Fe/S clusters Sulfurospirillum multivorans
1.21.99.5 iron-sulfur clusters two Fe/S clusters Sulfurospirillum multivorans
1.21.99.5 S two Fe/S clusters Sulfurospirillum multivorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.21.99.5 tetrachloroethene + reduced acceptor Sulfurospirillum multivorans
-
 trichloroethene + chloride + acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.21.99.5 Sulfurospirillum multivorans
-
 strictly anaerobic, gram-negative bacterium
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.99.5 1,1,3-trichloropropene + reduced acceptor 22.9% activity compared with perchloroethene as substrate Sulfurospirillum multivorans 1,1-dichloropropene + chloride + acceptor
-
 ?
1.21.99.5 2,3-dichloropropene + reduced acceptor 14.4% activity compared with perchloroethene as substrate Sulfurospirillum multivorans 2-chloropropene + chloride + acceptor
-
 ?
1.21.99.5 chlorinated propenes + reduced acceptor 22% of the reduction rates with perchloroethene Sulfurospirillum multivorans propene + chloride + acceptor
-
 ?
1.21.99.5 chloroacetamide + reduced acceptor very poor substrate Sulfurospirillum multivorans acetamide + chloride + acceptor
-
 ?
1.21.99.5 cis-1,3-dichloropropene + reduced acceptor chlorination rate is lower than for trans-1,3-dichloropropene and reaction ceases after 2-3 min Sulfurospirillum multivorans mono-chloropropene + chloride + acceptor
-
 ?
1.21.99.5 additional information substrate specificity Sulfurospirillum multivorans ?
-
 ?
1.21.99.5 additional information no activity with dichloroethene, 1-chloropropene, 2-chloropropene, 3-chloropropene, 1,1-dichloropropene, 1,2-dichloropropane, 1,3-dichloropropane, and 1,2,3-trichloropropane Sulfurospirillum multivorans ?
-
 ?
1.21.99.5 tetrachloroethene + reduced acceptor
-
 Sulfurospirillum multivorans trichloroethene + chloride + acceptor
-
 ?
1.21.99.5 tetrachloroethene + reduced acceptor besides reduced methyl viologen, titanium(III)citrate can serve as electron donor for reductive dehalogenation Sulfurospirillum multivorans trichloroethene + chloride + acceptor
-
 ?
1.21.99.5 tetrachloroethene + reduced acceptor perchloroethylene, perchloroethene, PCE Sulfurospirillum multivorans trichloroethene + chloride + acceptor
-
 ?
1.21.99.5 tetrachloromethane + reduced acceptor very poor substrate Sulfurospirillum multivorans ? + chloride + acceptor
-
 ?
1.21.99.5 trans-1,3-dichloropropene + reduced acceptor 5.7% activity compared with perchloroethene as substrate Sulfurospirillum multivorans mono-chloropropene + chloride + acceptor
-
 ?
1.21.99.5 trichloroethene + reduced acceptor besides reduced methyl viologen, titanium(III)citrate can serve as electron donor for reductive dehalogenation Sulfurospirillum multivorans cis-1,2-dichloroethene + chloride + acceptor
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.21.99.5 25
-
 assay at Sulfurospirillum multivorans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.21.99.5 1.5
-
 tetrachloroethene titanium(III)citrate as sole electron donor Sulfurospirillum multivorans
1.21.99.5 20.4
-
 tetrachloroethene methyl viologen as artificial electron donor Sulfurospirillum multivorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.21.99.5 7.5
-
 assay at Sulfurospirillum multivorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.21.99.5 corrinoid
-
 Sulfurospirillum multivorans