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Literature summary extracted from

  • Sreerama, L.; Sladek, N.E.
    Identification and characterization of a novel class 3 aldehyde dehydrogenase overexpressed in a human breast adenocarcinoma cell line exhibiting oxazaphosphorine-specific acquired resistance (1993), Biochem. Pharmacol., 45, 2487-2505.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.2.1.5 freezing and thawing, more than once causes complete loss of activity of enzyme from breast adenocarcinoma cell line, enzyme from stomach mucosa retains 40% of its activity even after being frozen and thawed eight times Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.5 0.054
-
 NAD+ reaction with benzaldehyde, enzyme from stomach mucosa Homo sapiens
1.2.1.5 0.067
-
 octanal reaction with NAD+ or NADP+, enzyme from breast adenocarcinoma cell line Homo sapiens
1.2.1.5 0.091
-
 4-Pyridinecarboxaldehyde reaction with NAD+ or NADP+, enzyme from breast adenocarcinoma cell line Homo sapiens
1.2.1.5 0.104
-
 octanal reaction with NADP+, enzyme from stomach mucosa Homo sapiens
1.2.1.5 0.113
-
 octanal reaction with NAD+, enzyme from stomach mucosa Homo sapiens
1.2.1.5 0.19
-
 4-Pyridinecarboxaldehyde reaction with NAD+ or NADP+, enzyme from stomach mucosa Homo sapiens
1.2.1.5 0.486
-
 benzaldehyde reaction with NADP+, enzyme from stomach mucosa Homo sapiens
1.2.1.5 0.505
-
 benzaldehyde reaction with NAD+, enzyme from stomach mucosa Homo sapiens
1.2.1.5 0.55
-
 NAD+ reaction with benzaldehyde, enzyme from adenocarcinoma cell line Homo sapiens
1.2.1.5 0.64
-
 benzaldehyde reaction with NAD+ or NADP+, enzyme from breast adenocarcinoma cell line Homo sapiens
1.2.1.5 0.64
-
 aldophosphamide reaction with NAD+ Homo sapiens
1.2.1.5 0.94
-
 NADP+ reaction with benzaldehyde, enzyme from stomach mucosa Homo sapiens
1.2.1.5 1
-
 NADP+ reaction with benzaldehyde, enzyme from adenocarcinoma cell line Homo sapiens
1.2.1.5 19
-
 propanal reaction with NADP+, enzyme from stomach mucosa Homo sapiens
1.2.1.5 19.06
-
 propanal reaction with NAD+, enzyme from stomach mucosa Homo sapiens
1.2.1.5 80
-
 acetaldehyde reaction with NAD+, enzyme from stomach mucosa Homo sapiens
1.2.1.5 81
-
 acetaldehyde reaction with NADP+, enzyme from stomach mucosa Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.5 40000
-
 2 * 40000, enzyme from breast adenocarcinoma cell line, SDS-PAGE Homo sapiens
1.2.1.5 54500
-
 2 * 54500, enzyme from stomach mucosa, SDS-PAGE Homo sapiens
1.2.1.5 108000
-
 enzyme from stomach mucosa, gel filtration Homo sapiens
1.2.1.5 110000
-
 non-denaturing PAGE Homo sapiens
1.2.1.5 125000
-
 enzyme from breast adenocarcinoma cell line, gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.5 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.5
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.5 breast adenocarcinoma cell exhibiting oxazaphosphorine-specific acquired resistance, enzyme form ALDH3 Homo sapiens
-
1.2.1.5 stomach mucosa Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.5 16.67
-
 enzyme from breast adenocarcinoma cell line Homo sapiens
1.2.1.5 32.95
-
 enzyme from stomach mucosa Homo sapiens

Storage Stability

EC Number Storage Stability Organism
1.2.1.5 4°C, pH 7.5, 25 mM sodium phosphate supplemented with 1 mM EDTA and 0.05% dithiothreitol, 10% glycerol, enzyme from breast adenocarcinoma cell line completely loses activity after 15 days, enzyme from stomach mucosa loses 75% of its activity after 3 months Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.5 4-pyridinecarboxaldehyde + NAD+ + H2O
-
 Homo sapiens 4-pyridinecarboxylate + NADH + H+
-
 ?
1.2.1.5 acetaldehyde + NAD+ + H2O activity with the enzyme from stomach mucosa, no activity with the enzyme from breast adenocarcinoma cell line Homo sapiens acetate + NADH + H+
-
 ?
1.2.1.5 aldophosphamide + NAD+
-
 Homo sapiens N,N-bis-(2-chloroethyl)-diaminophosphoric-(2-carboxyethylester) + ?
-
 ?
1.2.1.5 benzaldehyde + NAD+ + H2O
-
 Homo sapiens benzoate + NADH + H+
-
 ?
1.2.1.5 benzaldehyde + NADP+
-
 Homo sapiens benzoate + NADPH
-
 ?
1.2.1.5 octanal + NAD+ + H2O
-
 Homo sapiens octanoate + NADH + H+
-
 ?
1.2.1.5 propanal + NAD+ + H2O
-
 Homo sapiens propionate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.5 dimer 2 * 40000, enzyme from breast adenocarcinoma cell line, SDS-PAGE Homo sapiens
1.2.1.5 dimer 2 * 54500, enzyme from stomach mucosa, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.5 37
-
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.5 8 8.5 benzaldehyde oxidation, enzyme from breast adenocarcinoma cell line Homo sapiens
1.2.1.5 8 9 benzaldehyde oxidation, enzyme from stomach mucosa Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.2.1.5 6.5 7.5 highest stability of enzyme from breast adenocarcinoma cell line Homo sapiens
1.2.1.5 7.5 9.5 highest stability of enzyme from stomach mucosa Homo sapiens