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Literature summary extracted from

  • Bedino, S.; Testore, G.; Obert, F.
    Initial characterization of aldehyde dehydrogenase from rat testis cytosol (1990), Biol. Chem. Hoppe-Seyler, 371, 95-101.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.3 2-mercaptoethanol 100 mM, full activation Rattus norvegicus
1.2.1.3 dithiothreitol 4 mM, full activation Rattus norvegicus
1.2.1.3 GSH 30% activation Rattus norvegicus

General Stability

EC Number General Stability Organism
1.2.1.3 freezing causes loss of activity Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.3 benzaldehyde substrate inhibition Rattus norvegicus
1.2.1.3 Butanal substrate inhibition Rattus norvegicus
1.2.1.3 Ca2+ 0.4 mM, 50% inhibition Rattus norvegicus
1.2.1.3 D-glyceraldehyde substrate inhibition Rattus norvegicus
1.2.1.3 hexanal substrate inhibition Rattus norvegicus
1.2.1.3 Isobutanal substrate inhibition Rattus norvegicus
1.2.1.3 Mg2+ 0.4 mM, 50% inhibition Rattus norvegicus
1.2.1.3 pentanal substrate inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.3 0.004
-
 hexanal
-
 Rattus norvegicus
1.2.1.3 0.006
-
 benzaldehyde
-
 Rattus norvegicus
1.2.1.3 0.006
-
 pentanal
-
 Rattus norvegicus
1.2.1.3 0.007
-
 Butanal
-
 Rattus norvegicus
1.2.1.3 0.023
-
 propanal
-
 Rattus norvegicus
1.2.1.3 0.03
-
 Isobutanal
-
 Rattus norvegicus
1.2.1.3 0.05
-
 NAD+
-
 Rattus norvegicus
1.2.1.3 0.246
-
 acetaldehyde
-
 Rattus norvegicus
1.2.1.3 1.136
-
 D-glyceraldehyde
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.3 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.3
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.3 testis
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
1.2.1.3 4°C, phosphate buffer pH 7.4, containing 4 mM dithiothreitol or 10 mM 2-mercaptoethanol, stable for 8-10 days Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.3 acetaldehyde + NAD+ + H2O
-
 Rattus norvegicus acetate + NADH + H+
-
 ?
1.2.1.3 benzaldehyde + NAD+ + H2O
-
 Rattus norvegicus benzoate + NADH + H+
-
 ?
1.2.1.3 butanal + NAD+ + H2O
-
 Rattus norvegicus butyrate + NADH + H+
-
 ?
1.2.1.3 D-glyceraldehyde + NAD+ + H2O
-
 Rattus norvegicus D-glycerate + NADH + H+
-
 r
1.2.1.3 glyceraldehyde + NAD+ + H2O
-
 Rattus norvegicus glycerate + NADH
-
 ?
1.2.1.3 hexanal + NAD+ + H2O
-
 Rattus norvegicus hexanoate + NADH + H+
-
 ?
1.2.1.3 isobutanal + NAD+ + H2O
-
 Rattus norvegicus isobutyrate + NADH
-
 ?
1.2.1.3 pentanal + NAD+ + H2O
-
 Rattus norvegicus pentanoate + NADH
-
 ?
1.2.1.3 propanal + NAD+ + H2O
-
 Rattus norvegicus propionate + NADH
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.3 50
-
-
 Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.3 45
-
 90 min, pH 7.4, 0.2 M phosphate, 4 mM dithiothreitol, stable Rattus norvegicus
1.2.1.3 55
-
 30 min, 20% loss of activity of diluted solution, 70% loss of activity of concentrated preparations Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.3 8.5
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.3 7.3 9.5 50% of maximal activity at pH 7.3 and at pH 9.5 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.3 NAD+
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.1.3 0.17
-
 hexanal
-
 Rattus norvegicus
1.2.1.3 0.37
-
 benzaldehyde
-
 Rattus norvegicus
1.2.1.3 0.54
-
 pentanal
-
 Rattus norvegicus
1.2.1.3 4.35
-
 Butanal
-
 Rattus norvegicus
1.2.1.3 14.32
-
 D-glyceraldehyde
-
 Rattus norvegicus
1.2.1.3 100.1
-
 Isobutanal
-
 Rattus norvegicus