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Literature summary extracted from

  • Rout, U.K.; Weiner, H.
    Involvement of Serine 74 in the Enzyme-Coenzyme Interaction of Rat Liver Mitochondrial Aldehyde Dehydrogenase (1994), Biochemistry, 33, 8955-8961.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.3 S74A half-life of the mutant enzyme at 50°C is 1 min compared to the half-life of the native enzyme of 1 min. Mutation diminishes NAD+ binding, affecting both the on and the off rates, as well as the rate-limiting step. About 100fold higher Km-value for NAD+ Rattus norvegicus
1.2.1.3 S74C about 70fold higher Km-value for NAD+ Rattus norvegicus
1.2.1.3 S74T about 100fold higher Km-value for NAD+ Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.3 ADPribose competitive Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.3 0.0001
-
 benzaldehyde native enzyme Rattus norvegicus
1.2.1.3 0.0001
-
 p-nitrobenzaldehyde native enzyme Rattus norvegicus
1.2.1.3 0.0001
-
 p-Methoxybenzaldehyde native enzyme Rattus norvegicus
1.2.1.3 0.0001
-
 4-(dimethylamino)cinnamaldehyde native enzyme Rattus norvegicus
1.2.1.3 0.0002
-
 benzaldehyde mutant enzyme S74A Rattus norvegicus
1.2.1.3 0.0003
-
 4-(dimethylamino)cinnamaldehyde mutant enzyme S74A Rattus norvegicus
1.2.1.3 0.0004
-
 acetaldehyde native enzyme Rattus norvegicus
1.2.1.3 0.0004
-
 p-nitrobenzaldehyde mutant enzyme S74A Rattus norvegicus
1.2.1.3 0.0004
-
 p-Methoxybenzaldehyde mutant enzyme S74A Rattus norvegicus
1.2.1.3 0.0007
-
 propanal native enzyme Rattus norvegicus
1.2.1.3 0.0007
-
 Butanal native enzyme Rattus norvegicus
1.2.1.3 0.0008
-
 hexanal native enzyme Rattus norvegicus
1.2.1.3 0.0008
-
 propanal native enzyme and mutant enzyme S74A Rattus norvegicus
1.2.1.3 0.0009
-
 Glutaraldehyde native enzyme Rattus norvegicus
1.2.1.3 0.0009
-
 propanal mutant enzyme S74A Rattus norvegicus
1.2.1.3 0.002
-
 Butanal mutant enzyme S74A Rattus norvegicus
1.2.1.3 0.0024
-
 hexanal mutant enzyme S74A Rattus norvegicus
1.2.1.3 0.0027
-
 acetaldehyde mutant enzyme S74A Rattus norvegicus
1.2.1.3 0.003
-
 Glutaraldehyde mutant enzyme S74A Rattus norvegicus
1.2.1.3 0.01
-
 NAD+ native enzyme, reaction with propanal Rattus norvegicus
1.2.1.3 0.038
-
 glycolaldehyde native enzyme Rattus norvegicus
1.2.1.3 0.077
-
 glycolaldehyde mutant enzyme S74A Rattus norvegicus
1.2.1.3 0.178
-
 formaldehyde native enzyme Rattus norvegicus
1.2.1.3 0.7
-
 formaldehyde mutant enzyme S74A Rattus norvegicus
1.2.1.3 1.9
-
 NAD+ mutant enzyme S74A, reaction with propanal Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.3 mitochondrion
-
 Rattus norvegicus 5739
-

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.3 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.3 native and mutant enzyme S74A Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.3 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.3 acetaldehyde + NAD+ + H2O
-
 Rattus norvegicus acetate + NADH + H+
-
 ?
1.2.1.3 benzaldehyde + NAD+ + H2O
-
 Rattus norvegicus benzoate + NADH + H+
-
 ?
1.2.1.3 butanal + NAD+ + H2O
-
 Rattus norvegicus butyrate + NADH + H+
-
 ?
1.2.1.3 formaldehyde + NAD+ + H2O
-
 Rattus norvegicus formate + NADH
-
 ?
1.2.1.3 glutaraldehyde + NAD+ + H2O
-
 Rattus norvegicus glutarate + NADH
-
 ?
1.2.1.3 glycolaldehyde + NAD+ + H2O
-
 Rattus norvegicus glycolate + NADH
-
 ?
1.2.1.3 hexanal + NAD+ + H2O
-
 Rattus norvegicus hexanoate + NADH + H+
-
 ?
1.2.1.3 m-methoxybenzaldehyde + NAD+ + H2O
-
 Rattus norvegicus m-methoxybenzoate + NADH + H+
-
 ?
1.2.1.3 p-(dimethylamino)cinnamaldehyde + NAD+ + H2O
-
 Rattus norvegicus p-(dimethylamino)cinnamate + NADH
-
 ?
1.2.1.3 p-nitrobenzaldehyde + NAD+ + H2O
-
 Rattus norvegicus p-nitrobenzoate + NADH
-
 ?
1.2.1.3 propanal + NAD+ + H2O
-
 Rattus norvegicus propionate + NADH
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.3 37
-
 mutant enzyme S74A and native enzyme, stable Rattus norvegicus
1.2.1.3 50
-
 half-life of mutant enzyme S74A is 1 min, half-life of native enzyme is 4 min Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.3 additional information
-
 additional information
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.3 NAD+
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.1.3 0.32
-
 ADPribose native enzyme Rattus norvegicus
1.2.1.3 6.8
-
 ADPribose mutant enzyme S74A Rattus norvegicus