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Literature summary extracted from

  • Van Ophem, P.W.; Duine, J.A.
    Different types of formaldehyde-oxidizing dehydrogenases in Nocardia species 239: purification and characterization of an NAD-dependent aldehyde dehydrogenase (1990), Arch. Biochem. Biophys., 282, 248-253.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.2.1.3 freezing-thawing cycles do not affect activity Nocardia sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.3 1-butanol
-
 Nocardia sp.
1.2.1.3 1-propanol
-
 Nocardia sp.
1.2.1.3 acetaldehyde substrate inhibition Nocardia sp.
1.2.1.3 benzaldehyde substrate inhibition Nocardia sp.
1.2.1.3 ethanol
-
 Nocardia sp.
1.2.1.3 formaldehyde substrate inhibition Nocardia sp.
1.2.1.3 H2O2
-
 Nocardia sp.
1.2.1.3 HgCl2
-
 Nocardia sp.
1.2.1.3 methanol
-
 Nocardia sp.
1.2.1.3 PCMB
-
 Nocardia sp.
1.2.1.3 PMSF
-
 Nocardia sp.
1.2.1.3 propanal substrate inhibition Nocardia sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.3 0.006
-
 propanal
-
 Nocardia sp.
1.2.1.3 0.082
-
 acetaldehyde
-
 Nocardia sp.
1.2.1.3 0.33
-
 benzaldehyde
-
 Nocardia sp.
1.2.1.3 0.62
-
 NAD+ reaction with formaldehyde Nocardia sp.
1.2.1.3 0.65
-
 formaldehyde
-
 Nocardia sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.3 55000
-
 4 * 55000, SDS-PAGE Nocardia sp.
1.2.1.3 200000
-
 non-denaturing PAGE Nocardia sp.

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.3 Nocardia sp.
-
 239
-
1.2.1.3 Nocardia sp. 239
-
 239
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.3
-
 Nocardia sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.3 5.4
-
-
 Nocardia sp.

Storage Stability

EC Number Storage Stability Organism
1.2.1.3 -40°C, stable for several months Nocardia sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.3 acetaldehyde + NAD+ + H2O
-
 Nocardia sp. acetate + NADH + H+
-
 ?
1.2.1.3 acetaldehyde + NAD+ + H2O
-
 Nocardia sp. 239 acetate + NADH + H+
-
 ?
1.2.1.3 benzaldehyde + NAD+ + H2O
-
 Nocardia sp. benzoate + NADH + H+
-
 ?
1.2.1.3 benzaldehyde + NAD+ + H2O
-
 Nocardia sp. 239 benzoate + NADH + H+
-
 ?
1.2.1.3 formaldehyde + NAD+ + H2O
-
 Nocardia sp. formate + NADH
-
 ir
1.2.1.3 formaldehyde + NAD+ + H2O
-
 Nocardia sp. 239 formate + NADH
-
 ir
1.2.1.3 propanal + NAD+ + H2O
-
 Nocardia sp. propionate + NADH
-
 ?
1.2.1.3 propanal + NAD+ + H2O
-
 Nocardia sp. 239 propionate + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.3 tetramer 4 * 55000, SDS-PAGE Nocardia sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.3 37
-
-
 Nocardia sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.3 9.2
-
-
 Nocardia sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.2.1.3 7 8 stable Nocardia sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.3 additional information no activity with NADP+ Nocardia sp.
1.2.1.3 NAD+
-
 Nocardia sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.1.3 2
-
 benzaldehyde
-
 Nocardia sp.
1.2.1.3 2
-
 propanal
-
 Nocardia sp.
1.2.1.3 5
-
 acetaldehyde
-
 Nocardia sp.
1.2.1.3 6
-
 formaldehyde
-
 Nocardia sp.