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Literature summary extracted from

  • Nagasawa, T.; Utagawa, T.; Goto, J.; Kim, C.J.; Tani Y.; Kumagai, H.; Yamada, H.
    Syntheses of L-tyrosine-related amino acids by tyrosine phenol-lyase of Citrobacter intermedius (1981), Eur. J. Biochem., 117, 33-40.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.99.2 1,2-dihydroxybenzene cleavage of Tyr Citrobacter intermedius
4.1.99.2 1,3-dihydroxybenzene cleavage of Tyr Citrobacter intermedius
4.1.99.2 2-Aminophenol cleavage of Tyr Citrobacter intermedius
4.1.99.2 2-bromophenol cleavage of Tyr Citrobacter intermedius
4.1.99.2 2-chlorophenol cleavage of Tyr Citrobacter intermedius
4.1.99.2 2-Fluorophenol cleavage of Tyr Citrobacter intermedius
4.1.99.2 2-iodophenol cleavage of Tyr Citrobacter intermedius
4.1.99.2 2-methylphenol cleavage of Tyr Citrobacter intermedius
4.1.99.2 3-Aminophenol cleavage of Tyr Citrobacter intermedius
4.1.99.2 3-bromophenol cleavage of Tyr Citrobacter intermedius
4.1.99.2 3-chlorophenol cleavage of Tyr Citrobacter intermedius
4.1.99.2 3-Fluorophenol cleavage of Tyr Citrobacter intermedius
4.1.99.2 3-iodophenol cleavage of Tyr Citrobacter intermedius
4.1.99.2 3-methylphenol cleavage of Tyr Citrobacter intermedius
4.1.99.2 4-Aminophenol cleavage of Tyr Citrobacter intermedius
4.1.99.2 4-hydroxypyridine cleavage of Tyr Citrobacter intermedius
4.1.99.2 phenol cleavage of Tyr Citrobacter intermedius

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.99.2 0.21
-
 p-L-Tyr
-
 Citrobacter intermedius
4.1.99.2 0.35
-
 2-fluoro-L-Tyr
-
 Citrobacter intermedius
4.1.99.2 0.4
-
 3-fluoro-L-Tyr
-
 Citrobacter intermedius
4.1.99.2 0.7
-
 2-Chloro-L-Tyr
-
 Citrobacter intermedius
4.1.99.2 1.4
-
 3-bromo-L-Tyr
-
 Citrobacter intermedius
4.1.99.2 1.6
-
 2-methoxy-L-Tyr
-
 Citrobacter intermedius
4.1.99.2 2
-
 L-Phe
-
 Citrobacter intermedius
4.1.99.2 3
-
 3-Chloro-L-Tyr
-
 Citrobacter intermedius
4.1.99.2 3.5
-
 2-bromo-L-Tyr
-
 Citrobacter intermedius
4.1.99.2 3.7
-
 2-Methyl-L-Tyr
-
 Citrobacter intermedius
4.1.99.2 4.1
-
 3-methyl-L-Tyr
-
 Citrobacter intermedius

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.2 Citrobacter intermedius
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.99.2 partial Citrobacter intermedius

Reaction

EC Number Reaction Comment Organism Reaction ID
4.1.99.2 L-tyrosine + H2O = phenol + pyruvate + NH3 NH3 is the first substrate which interacts with bound pyridoxal 5'-phosphate, pyruvate is the second substrate Citrobacter intermedius
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.99.2 0.65
-
-
 Citrobacter intermedius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.2 2-bromo-L-Tyr + H2O
-
 Citrobacter intermedius 2-bromophenol + pyruvate + NH3
-
 r
4.1.99.2 2-bromophenol + pyruvate + NH3
-
 Citrobacter intermedius 2-bromo-L-Tyr + H2O
-
 r
4.1.99.2 2-chloro-L-Tyr + H2O
-
 Citrobacter intermedius 2-chlorophenol + pyruvate + NH3
-
 r
4.1.99.2 2-chlorophenol + pyruvate + NH3
-
 Citrobacter intermedius 2-chloro-L-Tyr + H2O
-
 r
4.1.99.2 2-fluoro-L-Tyr + H2O
-
 Citrobacter intermedius 2-fluorophenol + pyruvate + NH3
-
 r
4.1.99.2 2-fluorophenol + pyruvate + NH3
-
 Citrobacter intermedius 2-fluoro-L-Tyr + H2O
-
 r
4.1.99.2 2-methoxy-L-Tyr + H2O
-
 Citrobacter intermedius 2-methoxyphenol + pyruvate + NH3
-
 r
4.1.99.2 2-methoxyphenol + pyruvate + NH3
-
 Citrobacter intermedius 2-methoxy-L-Tyr + H2O
-
 r
4.1.99.2 2-methyl-L-Tyr + H2O
-
 Citrobacter intermedius 2-methylphenol + pyruvate + NH3
-
 r
4.1.99.2 2-methylphenol + pyruvate + NH3
-
 Citrobacter intermedius 2-methyl-L-Tyr + H2O
-
 r
4.1.99.2 3-bromo-L-Tyr + H2O
-
 Citrobacter intermedius 3-bromophenol + pyruvate + NH3
-
 r
4.1.99.2 3-bromophenol + pyruvate + NH3
-
 Citrobacter intermedius 3-bromo-L-Tyr + H2O
-
 r
4.1.99.2 3-chloro-L-Tyr + H2O
-
 Citrobacter intermedius 3-chlorophenol + pyruvate + NH3
-
 r
4.1.99.2 3-chlorophenol + pyruvate + NH3
-
 Citrobacter intermedius 3-chloro-L-Tyr + H2O
-
 r
4.1.99.2 3-fluoro-L-Tyr + H2O
-
 Citrobacter intermedius 3-fluorophenol + pyruvate + NH3
-
 r
4.1.99.2 3-fluorophenol + pyruvate + NH3
-
 Citrobacter intermedius 3-fluoro-L-Tyr + H2O
-
 r
4.1.99.2 3-methyl-L-Tyr + H2O
-
 Citrobacter intermedius 3-methylphenol + pyruvate + NH3
-
 r
4.1.99.2 3-methylphenol + pyruvate + NH3
-
 Citrobacter intermedius 3-methyl-L-Tyr + H2O
-
 r
4.1.99.2 L-Phe + H2O
-
 Citrobacter intermedius ?
-
 r
4.1.99.2 phenol + pyruvate + NH3
-
 Citrobacter intermedius Tyr + H2O
-
 r
4.1.99.2 Tyr + H2O L-Tyr Citrobacter intermedius phenol + pyruvate + NH3
-
 r

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.99.2 pyridoxal 5'-phosphate cofactor Citrobacter intermedius