Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Parkin, D.W.
    Purine-specific nucleoside N-ribohydrolase from Trypanosoma brucei brucei (1996), J. Biol. Chem., 271, 21713-21719.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.1 3-deazaadenosine
-
 Trypanosoma brucei
3.2.2.1 7-deazaadenosine i.e. tubercidin Trypanosoma brucei
3.2.2.1 D-ribose
-
 Trypanosoma brucei
3.2.2.1 formycin B
-
 Trypanosoma brucei
3.2.2.1 hypoxanthine
-
 Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.1 0.015
-
 adenosine
-
 Trypanosoma brucei
3.2.2.1 0.018
-
 Inosine
-
 Trypanosoma brucei
3.2.2.1 0.019
-
 purine riboside
-
 Trypanosoma brucei
3.2.2.1 0.026
-
 5-methyluridine
-
 Trypanosoma brucei
3.2.2.1 0.042
-
 2'-deoxyadenosine
-
 Trypanosoma brucei
3.2.2.1 0.046
-
 guanosine
-
 Trypanosoma brucei
3.2.2.1 0.102
-
 uridine
-
 Trypanosoma brucei
3.2.2.1 0.106
-
 cytidine
-
 Trypanosoma brucei
3.2.2.1 0.562
-
 p-nitrophenylriboside
-
 Trypanosoma brucei
3.2.2.1 0.6
-
 3'-deoxyadenosine
-
 Trypanosoma brucei
3.2.2.1 9.87
-
 5'-deoxyadenosine
-
 Trypanosoma brucei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.1 35759
-
 2 * 35759, electrospray ionization mass spectrometry Trypanosoma brucei
3.2.2.1 41000
-
 2 * 41000, SDS-PAGE Trypanosoma brucei
3.2.2.1 71000
-
 gel filtration Trypanosoma brucei

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.1 Trypanosoma brucei
-
 brucei
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.1
-
 Trypanosoma brucei

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.2.1 bloodstream form
-
 Trypanosoma brucei
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.1 50
-
 hydrolysis of inosine Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.1 2'-deoxyadenosine + H2O
-
 Trypanosoma brucei D-ribose + ?
-
 ?
3.2.2.1 3'-deoxyadenosine + H2O
-
 Trypanosoma brucei D-ribose + ?
-
 ?
3.2.2.1 5'-deoxyadenosine + H2O
-
 Trypanosoma brucei D-ribose + ?
-
 ?
3.2.2.1 5-methyluridine + H2O
-
 Trypanosoma brucei 5-methyluracil + D-ribose
-
 ?
3.2.2.1 adenosine + H2O
-
 Trypanosoma brucei D-ribose + adenine
-
 ?
3.2.2.1 cytidine + H2O
-
 Trypanosoma brucei cytosine + D-ribose
-
 r
3.2.2.1 guanosine + H2O
-
 Trypanosoma brucei D-ribose + guanine
-
 ?
3.2.2.1 inosine + H2O kinetic mechanism is rapid equilibrium with random product release Trypanosoma brucei hypoxanthine + D-ribose
-
 ?
3.2.2.1 p-nitrophenylriboside + H2O
-
 Trypanosoma brucei 4-nitrophenol + D-ribose
-
 ?
3.2.2.1 purine riboside + H2O
-
 Trypanosoma brucei purine + D-ribose
-
 ?
3.2.2.1 uridine + H2O
-
 Trypanosoma brucei D-ribose + uracil
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.2.1 dimer 2 * 41000, SDS-PAGE Trypanosoma brucei
3.2.2.1 dimer 2 * 35759, electrospray ionization mass spectrometry Trypanosoma brucei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.2.1 34
-
 Inosine
-
 Trypanosoma brucei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.1 7
-
-
 Trypanosoma brucei

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.2.1 5 10 stable Trypanosoma brucei