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Literature summary extracted from

  • Levy-Schil, S.; Soubrier, F.; Crutz-Le Coq, A.M.; Faucher, D.; Crouzet, J.; Petre, D.
    Aliphatic nitrilase from a soil-isolated Comamonas testosteroni sp.: gene cloning and overexpression, purification and primary structure (1995), Gene, 161, 15-20.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.5.1 synthesis nitrilase products are intermediates in the synthesis of nylon Comamonas testosteroni

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.5.1 expression in Escherichia coli Comamonas testosteroni
3.5.5.7 expression in Escherichia coli Comamonas testosteroni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.5.1 acrylonitrile + H2O Comamonas testosteroni
-
 acrylic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O Comamonas testosteroni low activity benzoic acid + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.1 Comamonas testosteroni Q59329
-
-
3.5.5.7 Comamonas testosteroni
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.5.1 to homogeneity, recombinant enzyme Comamonas testosteroni
3.5.5.7
-
 Comamonas testosteroni

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.5.1 additional information nitA gene Comamonas testosteroni
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.5.1 68
-
-
 Comamonas testosteroni
3.5.5.7 68
-
-
 Comamonas testosteroni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.1 acrylonitrile + H2O
-
 Comamonas testosteroni acrylic acid + NH3
-
 ?
3.5.5.1 adiponitrile + H2O preferred substrate, specific for aliphatic nitriles Comamonas testosteroni adipic acid + NH3
-
 ?
3.5.5.1 adiponitrile + H2O no formation of amide Comamonas testosteroni adipic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O low activity Comamonas testosteroni benzoic acid + NH3
-
 ?
3.5.5.1 cyano-5-valeramide + H2O
-
 Comamonas testosteroni valeramide + NH3
-
 ?
3.5.5.1 cyano-5-valeric acid + H2O
-
 Comamonas testosteroni valeric acid + NH3
-
 ?
3.5.5.1 propionitrile + H2O low activity Comamonas testosteroni propionic acid + NH3
-
 ?
3.5.5.7 acrylonitrile + H2O at 23% of the activity with adiponitrile Comamonas testosteroni acrylic acid + NH3
-
 ?
3.5.5.7 adiponitrile + H2O
-
 Comamonas testosteroni adipic acid + NH3
-
 ?
3.5.5.7 benzonitrile + 2 H2O at 4% of the activity with acrylonitrile Comamonas testosteroni benzoate + NH3
-
 ?
3.5.5.7 cyano-5-valeramide + H2O at 28% of the activity with adiponitrile Comamonas testosteroni glutaric acid 5-amide + NH3
-
 ?
3.5.5.7 cyano-5-valeric acid + H2O + H2O at 28% of the activity with adiponitrile Comamonas testosteroni ?
-
 ?
3.5.5.7 propionitrile + H2O at 6% of the activity with acrylonitrile Comamonas testosteroni propionate + NH3
-
 ?