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Literature summary extracted from

  • Schiavo, G.; Benfenati, F.; Poulain, B.; Rossetto, O.; Polverino de Laureto, P.; DasGupta, B.R.; Montecucco, C.
    Tetanus and botulinum-B neurotoxins block neurotransmitter release by proteolytic cleavage of synaptobrevin (1992), Nature, 359, 832-835.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.68 Ala-Ser-Gln-Phe-Glu-Thr-Ser synthetic peptide containing cleavage site of synaptobrevin, inhibits toxin action on buccal ganglion of Aplysia californica Clostridium tetani
3.4.24.68 captopril
-
 Clostridium tetani
3.4.24.68 EDTA
-
 Clostridium tetani
3.4.24.68 Gln-Phe-Glu-Thr synthetic peptide containing cleavage site of synaptobrevin, inhibits toxin action on buccal ganglion of Aplysia californica Clostridium tetani
3.4.24.69 Ala-Ser-Gln-Phe-Glu-Thr-Ser synthetic peptide containing cleavage site of synaptobrevin, inhibits toxin action on buccal ganglion of Aplysia californica, serotype BoNT/B, not A or E Clostridium botulinum
3.4.24.69 captopril serotype BoNT/B Clostridium botulinum
3.4.24.69 EDTA serotype BoNT/B Clostridium botulinum
3.4.24.69 Gln-Phe-Glu-Thr synthetic peptide containing cleavage site of synaptobrevin, inhibits toxin action on buccal ganglion of Aplysia californica, serotype BoNT/B, not A or E Clostridium botulinum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.68 Zinc zinc-dependent endoproteinase Clostridium tetani
3.4.24.69 Zinc activation requires reduction of interchain disulfide bond Clostridium botulinum
3.4.24.69 Zinc zinc-dependent endopeptidase (serotype BoNT/B) Clostridium botulinum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.68 synaptobrevin + H2O Clostridium tetani enzyme disables neuroexocytosis apparatus, acts at spinal inhibitory interneurons, blocking release of various neurotransmitters to produce spastic paralysis, clostridial neurotoxins are described as the most toxic substances known ?
-
 ?
3.4.24.68 synaptobrevin + H2O Clostridium tetani neurotoxin blocks neurotransmitter release in Aplysia neurons ?
-
 ?
3.4.24.69 synaptobrevin + H2O Clostridium botulinum
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.68 Clostridium tetani
-
-
-
3.4.24.69 Clostridium botulinum
-
 serotypes BoNT/A, BoNT/B
-
3.4.24.69 Clostridium botulinum
-
 BoNT/E
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.24.69 Limited hydrolysis of proteins of the neuroexocytosis apparatus, synaptobrevin (also known as neuronal vesicle-associated membrane protein, VAMP), synaptosome-associated protein of 25 kDa (SNAP25) or syntaxin. No detected action on small molecule substrates mechanism Clostridium botulinum
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.68 additional information catalytic activity requires reduction of the single interchain disulfide bond of the neurotoxin Clostridium tetani ?
-
 ?
3.4.24.68 additional information no substrates are rat or chicken Clostridium tetani ?
-
 ?
3.4.24.68 Synaptobrevin + H2O i.e. VAMP, neuronal vesicle-associated membrane protein, MW 19000, with 2 isoforms in human, chicken, in rat brain: synaptobrevin/VAMP-1 and synaptobrevin/VAMP-2, cleaves at Gln76-Phe77, the same site as botulin neurotoxin B Clostridium tetani Hydrolyzed synaptobrevin
-
 ?
3.4.24.68 synaptobrevin + H2O enzyme disables neuroexocytosis apparatus, acts at spinal inhibitory interneurons, blocking release of various neurotransmitters to produce spastic paralysis, clostridial neurotoxins are described as the most toxic substances known Clostridium tetani ?
-
 ?
3.4.24.68 synaptobrevin + H2O neurotoxin blocks neurotransmitter release in Aplysia neurons Clostridium tetani ?
-
 ?
3.4.24.69 additional information catalytic activity requires reduction of the single interchain disulfide bond of the neurotoxin Clostridium botulinum ?
-
 ?
3.4.24.69 additional information activating protease activity is localized on light or L-chain of neurotoxin Clostridium botulinum ?
-
 ?
3.4.24.69 Synaptobrevin + H2O hydrolyzed by serotypes BoNT/B Clostridium botulinum Hydrolyzed synaptobrevin
-
 ?
3.4.24.69 Synaptobrevin + H2O serotype BoNT/B: cleavage at Gln76-Phe77 Clostridium botulinum Hydrolyzed synaptobrevin
-
 ?
3.4.24.69 Synaptobrevin + H2O no substrate of serotype BoNT/A or E Clostridium botulinum Hydrolyzed synaptobrevin
-
 ?
3.4.24.69 Synaptobrevin + H2O carrying synaptobrevin/VAMP-2 Clostridium botulinum Hydrolyzed synaptobrevin
-
 ?
3.4.24.69 Synaptobrevin + H2O carrying Val76 instead of Gln76 is not hydrolyzed by serotype BoNT/B Clostridium botulinum Hydrolyzed synaptobrevin
-
 ?
3.4.24.69 Synaptobrevin + H2O highly specific neurotoxins Clostridium botulinum Hydrolyzed synaptobrevin
-
 ?
3.4.24.69 synaptobrevin + H2O
-
 Clostridium botulinum ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.68 37
-
 assay at Clostridium tetani
3.4.24.69 37
-
 assay at Clostridium botulinum