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Literature summary extracted from

  • Lauvergeat, V.; Lacomme, C.; Lacombe, E.; Lasserre, E.; Roby, D.; Grima-Pettenati, J.
    Two cinnamoyl-CoA reductase (CCR) genes from Arabidopsis thaliana are differentially expressed during development and in response to infection with pathogenic bacteria (2001), Phytochemistry, 57, 1187-1195.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.44 two genes AtCCR1 and AtCCR2, localised on chromosome 1, AtCCR1 is associated with lignin biosynthesis during development, AtCCR2 is associated with the hypersensitive response to pathogen attack Arabidopsis thaliana
1.2.1.44 two recombinant proteins expressed in Escherichia coli Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.44 0.00098
-
 feruloyl-CoA recombinant fusion protein GST-AtCCR1 Arabidopsis thaliana
1.2.1.44 0.0047
-
 feruloyl-CoA recombinant fusion protein GST-AtCCR2 Arabidopsis thaliana
1.2.1.44 0.00516
-
 caffeoyl-CoA recombinant fusion protein GST-AtCCR2 Arabidopsis thaliana
1.2.1.44 0.00632
-
 sinapoyl-CoA recombinant fusion protein GST-AtCCR1 Arabidopsis thaliana
1.2.1.44 0.0125
-
 caffeoyl-CoA recombinant fusion protein GST-AtCCR1 Arabidopsis thaliana
1.2.1.44 0.0307
-
 sinapoyl-CoA recombinant fusion protein GST-AtCCR2 Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.44 36600
-
 AtCCR2, predicted from gene sequence Arabidopsis thaliana
1.2.1.44 37500
-
 AtCCR1, predicted from gene sequence Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.44 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.44 recombinant fusion proteins GST-AtCCR1 and GST-AtCCR2, expressed in Escherichia coli Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.44 flower AtCCR1, not AtCCR 2, northern blot analysis Arabidopsis thaliana
-
1.2.1.44 leaf AtCCR1, not AtCCR2, northern blot analysis Arabidopsis thaliana
-
1.2.1.44 leaf strong and transient increase of AtCCR2 mRNA after inoculation with Xanthomonas campestris pv. campestris, northern blot analysis Arabidopsis thaliana
-
1.2.1.44 stem AtCCR1, not AtCCR2, northern blot analysis Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.44 caffeoyl-CoA + NADPH
-
 Arabidopsis thaliana caffeic aldehyde + CoA + NADP+
-
 ?
1.2.1.44 cinnamoyl-CoA + NADPH
-
 Arabidopsis thaliana cinnamaldehyde + CoA + NADP+
-
 ?
1.2.1.44 feruloyl-CoA + NADPH
-
 Arabidopsis thaliana ferulic aldehyde + CoA + NADP+
-
 ?
1.2.1.44 sinapoyl-CoA + NADPH
-
 Arabidopsis thaliana sinapic aldehyde + CoA + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.44 CCR ambiguous Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.44 0.0145
-
 caffeoyl-CoA recombinant fusion protein GST-AtCCR2 Arabidopsis thaliana
1.2.1.44 0.0363
-
 caffeoyl-CoA recombinant fusion protein GST-AtCCR1 Arabidopsis thaliana
1.2.1.44 0.0372
-
 feruloyl-CoA recombinant fusion protein GST-AtCCR2 Arabidopsis thaliana
1.2.1.44 0.038
-
 feruloyl-CoA recombinant fusion protein GST-AtCCR1 Arabidopsis thaliana
1.2.1.44 0.0583
-
 sinapoyl-CoA recombinant fusion protein GST-AtCCR2 Arabidopsis thaliana
1.2.1.44 0.0612
-
 sinapoyl-CoA recombinant fusion protein GST-AtCCR1 Arabidopsis thaliana