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Literature summary extracted from

  • Sarni, F.; Grand, C.; Boudet, A.M.
    Purification and properties of cinnamoyl-CoA reductase and cinnamyl alcohol dehydrogenase from poplar stems (Populus X euramericana) (1984), Eur. J. Biochem., 139, 259-265.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.44 2-mercaptoethanol
-
 Populus x canadensis
1.2.1.44 bovine serum albumin twofold increase of enzyme activity Populus x canadensis
1.2.1.44 dithiothreitol
-
 Populus x canadensis

General Stability

EC Number General Stability Organism
1.1.1.195 2-mercaptoethanol stabilizes Populus x canadensis
1.1.1.195 60% loss of activity after 20 h incubation in buffer containing a divalent cation-exchange resin Populus x canadensis
1.1.1.195 dithiothreitol stabilizes Populus x canadensis
1.1.1.195 ethylene glycol stabilizes Populus x canadensis
1.2.1.44 2-mercaptoethanol: stabilizes , only stable in presence of Populus x canadensis
1.2.1.44 ethyleneglycol, 15%, stabilizes Populus x canadensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.44 1,10-phenanthroline
-
 Populus x canadensis
1.2.1.44 4-chloromercuribenzoate
-
 Populus x canadensis
1.2.1.44 N-ethylmaleimide
-
 Populus x canadensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.195 0.00077
-
 coniferaldehyde
-
 Populus x canadensis
1.1.1.195 0.0012
-
 p-coumaraldehyde
-
 Populus x canadensis
1.1.1.195 0.0048
-
 sinapaldehyde
-
 Populus x canadensis
1.1.1.195 0.008
-
 NADPH
-
 Populus x canadensis
1.2.1.44 0.00235
-
 feruloyl-CoA
-
 Populus x canadensis
1.2.1.44 0.0037
-
 sinapoyl-CoA
-
 Populus x canadensis
1.2.1.44 0.00427
-
 p-Coumaroyl-CoA
-
 Populus x canadensis
1.2.1.44 0.0116
-
 NADPH cosubstrate sinapoyl-CoA Populus x canadensis
1.2.1.44 0.0143
-
 NADPH cosubstrate p-coumaroyl-CoA Populus x canadensis
1.2.1.44 0.0188
-
 NADPH cosubstrate feruloyl-CoA Populus x canadensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.195 Zn2+ restores activity after incubation in buffer containing a divalent-cation exchange resin Populus x canadensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.195 40000
-
 2 * 40000, SDS-PAGE Populus x canadensis
1.1.1.195 70000
-
 gel filtration Populus x canadensis
1.2.1.44 36000
-
 gel filtration Populus x canadensis
1.2.1.44 38000
-
 1 * 38000, SDS-PAGE Populus x canadensis

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.195 Populus x canadensis
-
-
-
1.2.1.44 Populus x canadensis
-
 poplar
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.195
-
 Populus x canadensis
1.2.1.44 poplar stem Populus x canadensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.44 stem xylem, sclerenchyma Populus x canadensis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.195 1.71
-
-
 Populus x canadensis
1.2.1.44 1.78
-
 with feruloyl-CoA as substrate Populus x canadensis

Storage Stability

EC Number Storage Stability Organism
1.1.1.195 4°C, mercaptoethanol, ethylene glycol, 1 month Populus x canadensis
1.2.1.44 4°C, mercaptoethanol, ethyleneglycol, 1 month, 35% loss of activity Populus x canadensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.195 coniferyl alcohol + NADP+
-
 Populus x canadensis coniferyl aldehyde + NADPH + H+
-
 ?
1.1.1.195 p-coumaryl alcohol + NADP+
-
 Populus x canadensis p-coumaraldehyde + NADPH
-
 ?
1.1.1.195 sinapyl alcohol + NADP+
-
 Populus x canadensis sinapaldehyde + NADPH
-
 ?
1.2.1.44 cinnamoyl-CoA + NADPH catalyzes preferentially the formation of cinnamaldehyde Populus x canadensis cinnamaldehyde + CoA + NADP+ catalyzes preferentially the formation of cinnamaldehyde ?
1.2.1.44 feruloyl-CoA + NADPH
-
 Populus x canadensis ferulic aldehyde + CoA + NADP+
-
 r
1.2.1.44 p-coumaroyl-CoA + NADPH
-
 Populus x canadensis p-coumaric aldehyde + CoA + NADP+
-
 ?
1.2.1.44 sinapoyl-CoA + NADPH
-
 Populus x canadensis sinapic aldehyde + CoA + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.195 dimer 2 * 40000, SDS-PAGE Populus x canadensis
1.2.1.44 monomer 1 * 38000, SDS-PAGE Populus x canadensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.44 30
-
 assay at Populus x canadensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.195 6
-
 reduction of coniferyl aldehyde, KH2PO4/Na2HPO4 buffer Populus x canadensis
1.1.1.195 7
-
 reduction of coniferyl aldehyde, Tris maleate/NaOH buffer Populus x canadensis
1.2.1.44 6 6.5
-
 Populus x canadensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.44 additional information
-
-
 Populus x canadensis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.195 7.5
-
 4°C, 1 week, 50% loss of activity Populus x canadensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.195 NADP+
-
 Populus x canadensis
1.2.1.44 NADP+ not: NADH Populus x canadensis