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Literature summary extracted from

  • Li, T.; Rosazza, J.P.N.
    Purification, characterization, and properties of an aryl aldehyde oxidoreductase from Nocardia sp. strain NRRL 5646 (1997), J. Bacteriol., 179, 3482-3487.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.2.1.30 glycerol in chromatographic buffers stabilizes Nocadia sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.30 anthranilic acid competitive inhibitor with benzoic acid as a substrate Nocadia sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.30 0.0293
-
 NADPH
-
 Nocadia sp.
1.2.1.30 0.0345
-
 (R)-ibuprofen
-
 Nocadia sp.
1.2.1.30 0.0573
-
 ATP
-
 Nocadia sp.
1.2.1.30 0.155
-
 Ibuprofen
-
 Nocadia sp.
1.2.1.30 0.645
-
 benzoate
-
 Nocadia sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.30 140000
-
 1 * 140000, SDS-PAGE Nocadia sp.
1.2.1.30 163000
-
 gel filtration Nocadia sp.

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.30 Nocadia sp.
-
-
-
1.2.1.30 Nocadia sp. NRRL 5646
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.30 by a combination of Mono-Q, Reactive Green 19 agarose affinity chromatography, and hydroxyapatite chromatography Nocadia sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.30 5.89
-
 purified enzyme Nocadia sp.

Storage Stability

EC Number Storage Stability Organism
1.2.1.30 4°C, 50 mM Tris-HCl buffer, pH 7.5, 10% glycerol, 8 hours Nocadia sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.30 (R)-ibuprofen + NADPH + ATP
-
 Nocadia sp. (2R)-2-(4-(2-methylpropyl)phenyl)propanal + NADP+ + AMP + H2O
-
 ?
1.2.1.30 (R)-ibuprofen + NADPH + ATP
-
 Nocadia sp. NRRL 5646 (2R)-2-(4-(2-methylpropyl)phenyl)propanal + NADP+ + AMP + H2O
-
 ?
1.2.1.30 aromatic acids + NADPH + H+ + ATP
-
 Nocadia sp. aromatic aldehydes + NADP+ + AMP + diphosphate + H2O
-
 ir
1.2.1.30 aromatic acids + NADPH + H+ + ATP
-
 Nocadia sp. NRRL 5646 aromatic aldehydes + NADP+ + AMP + diphosphate + H2O
-
 ir
1.2.1.30 benzoic acid + NADPH + H+ + ATP the reaction proceeds via benzoyladenosine 5'-monophosphate which is further reduced by NADPH to benzaldehyde Nocadia sp. benzaldehyde + NADP+ + AMP + diphosphate
-
 ?
1.2.1.30 benzoic acid + NADPH + H+ + ATP the reaction proceeds via benzoyladenosine 5'-monophosphate which is further reduced by NADPH to benzaldehyde Nocadia sp. NRRL 5646 benzaldehyde + NADP+ + AMP + diphosphate
-
 ?
1.2.1.30 ibuprofen + NADPH + ATP
-
 Nocadia sp. 2-(4-isobutylphenyl)propanal + NADP+ + AMP + H2O
-
 ?
1.2.1.30 ibuprofen + NADPH + ATP
-
 Nocadia sp. NRRL 5646 2-(4-isobutylphenyl)propanal + NADP+ + AMP + H2O
-
 ?
1.2.1.30 additional information other substrates are phenyl-substituted aliphatic acids, heterocyclic carboxylic acids, polyaromatic ring carboxylic acids, ibuprofen and its (R)-(-) isomer Nocadia sp. ?
-
 ?
1.2.1.30 additional information other substrates are phenyl-substituted aliphatic acids, heterocyclic carboxylic acids, polyaromatic ring carboxylic acids, ibuprofen and its (R)-(-) isomer Nocadia sp. NRRL 5646 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.30 monomer 1 * 140000, SDS-PAGE Nocadia sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.30 ATP
-
 Nocadia sp.
1.2.1.30 NADPH
-
 Nocadia sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.1.30 0.261
-
 anthranilic acid
-
 Nocadia sp.